Kothalawala Shashika Dinethri, Edward Daniya, Harasgama Jayamini C, Ranaweera Loshini, Weerasena Ovitigala Vithanage Don Sisira Jagathpriya, Niloofa Roshan, Ratnasooriya Wanigasekera Daya, Premakumara Galbada Arachchige Sirimal, Handunnetti Shiroma M
Institute of Biochemistry, Molecular Biology and Biotechnology (IBMBB), University of Colombo, Colombo, Sri Lanka.
Department of Zoology and Environmental Science, Faculty of Science, University of Colombo, Colombo, Sri Lanka.
Evid Based Complement Alternat Med. 2020 Sep 14;2020:9715060. doi: 10.1155/2020/9715060. eCollection 2020.
To investigate the immunomodulatory activity of a traditional Sri Lankan concoction of L. and (Gaertn.) Colebr., which is a Sri Lankan traditional medicine used to relieve inflammation and cold.
anti-inflammatory activity was tested using carrageenan-induced rat paw-edema model. Mechanism of anti-inflammatory activity was assessed by investigating the production of nitric oxide (NO), expression of iNOS enzyme, and reactive oxygen species (ROS) by rat peritoneal cells. The membrane stabilizing activity was also tested. The antibody response was determined by assessing the specific haemagglutination antibodies raised against sheep red blood cells.
The three doses of freeze-dried concoction used ((human equivalent dose (HED)-183 mg/kg) 2 × HED and 1/2HED; = 6 rats/group) showed significant inhibition of paw edema compared to water control at 3-5 hours ( < 0.05). Both HED and 1/2HED exhibited marked anti-inflammatory activity (72-83% inhibition at 4-5 hours; < 0.05). The HED of the concoction showed significant inhibition of NO (77.5 ± 0.73%, < 0.001) and ROS production (26.9 ± 2.55%; < 0.01) by rat peritoneal cells. Inhibition of NO production in the concoction treated rat peritoneal cells was confirmed by the lack of iNOS expression. The concoction also exhibited significant membrane stabilizing activity (IC = 0.0006 g/ml; = 0.001). HED resulted in a significantly high induction of specific antibody production against SRBC antigens as detected by SRBC haemagglutination assay (mean day 14 titers 253.3 compared to control: 66.7) ( < 0.01).
The traditional Sri Lankan concoction of and demonstrated potent anti-inflammatory activity, significant reduction of ROS, and NO production by rat peritoneal cells and the lack of iNOS expression confirmed the low NO production. The increased membrane stability also supports the anti-inflammatory activity of the concoction. Further, this concoction induced a significantly high antibody response reflecting its immunostimulatory activity. Together these results scientifically validate the therapeutic use of the concoction of and in Sri Lankan traditional medicinal system for immunomodulatory effects.
研究斯里兰卡传统草药组合罗勒（Ocimum basilicum L.）和长柄胡椒（Piper longum (Gaertn.) Colebr.）的免疫调节活性，该组合是一种用于缓解炎症和感冒的斯里兰卡传统药物。
使用角叉菜胶诱导的大鼠足趾肿胀模型测试抗炎活性。通过研究大鼠腹腔细胞中一氧化氮（NO）的产生、诱导型一氧化氮合酶（iNOS）的表达和活性氧（ROS）来评估抗炎活性的机制。还测试了膜稳定活性。通过评估针对绵羊红细胞产生的特异性血凝抗体来确定抗体反应。
所用的三种冻干草药组合剂量（人体等效剂量（HED）-183 mg/kg、2×HED和1/2HED；每组n = 6只大鼠）在3 - 5小时时与水对照组相比，对足趾肿胀有显著抑制作用（P < 0.05）。HED和1/2HED均表现出显著的抗炎活性（4 - 5小时时抑制率为72 - 83%；P < 0.05）。该草药组合的HED对大鼠腹腔细胞产生的NO（77.5 ± 0.73%，P < 极小值）和ROS（26.9 ± 2.55%；P < 0.01）有显著抑制作用。通过缺乏iNOS表达证实了该草药组合处理的大鼠腹腔细胞中NO产生的抑制。该草药组合还表现出显著的膜稳定活性（IC50 = 0.0006 g/ml；P = 0.001）。通过SRBC血凝试验检测，HED导致针对SRBC抗原产生的特异性抗体产生显著高诱导（第14天平均滴度为253.3，而对照组为66.7）（P < 0.01）。
斯里兰卡传统草药组合罗勒和长柄胡椒表现出强大的抗炎活性，能显著降低大鼠腹腔细胞中ROS和NO的产生，且缺乏iNOS表达证实了低NO产生。膜稳定性的增加也支持了该草药组合的抗炎活性。此外，该草药组合诱导了显著高的抗体反应，反映了其免疫刺激活性。这些结果共同从科学上验证了罗勒和长柄胡椒组合在斯里兰卡传统医学系统中用于免疫调节作用的治疗用途。
