印度防己醇提物对人结肠癌细胞的体外抗增殖活性
In vitro anti-proliferative activity of alcoholic stem extract of Coscinium fenestratum in human colorectal cancer cells.
作者信息
Rojsanga Piyanuch, Sukhthankar Mugdha, Krisanapun Chutwadee, Gritsanapan Wandee, Lawson Darunee Buripakdi, Baek Seung Joon
机构信息
Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, Knoxville, TN, USA ; ; Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok;
出版信息
Exp Ther Med. 2010 Jan;1(1):181-186. doi: 10.3892/etm_00000029. Epub 2010 Jan 1.
Coscinium fenestratum (Gaertn.) Colebr. is traditionally used for the treatment of cancer, arthritis and diabetes mellitus. The purpose of this study was to determine the molecular mechanisms by which this plant shows beneficial effects. An 80% ethanolic extract of C. fenestratum (80ET) was separated by its polarity into dichloromethane (DCM) and aqueous fractions (WF), and the anti-proliferative effects of 80ET, DCM and WF were investigated. Berberine, one of the major components of C. fenestratum, was used as a control. The 80ET, DCM, WF and berberine showed anti-proliferative activity as assessed by cell growth assay. Subsequently, the pro-apoptotic proteins NAG-1 and ATF3 were increased and the cell cycle protein cyclin D1 was decreased by the extract and its fractions. Interestingly, only the DCM fraction exhibited the induction of peroxisome proliferator-activated receptor γ (PPARγ) binding activity, which represents a pro-apoptotic activity in colorectal cancer cells. The overall results of this study indicate that the extract from this plant has anti-proliferative activity through the activation of pro-apoptotic proteins and PPARγ, and may have potential as a preventive regimen in the treatment of cancer.
箭叶风车子（Coscinium fenestratum (Gaertn.) Colebr.）传统上用于治疗癌症、关节炎和糖尿病。本研究的目的是确定该植物显示有益效果的分子机制。箭叶风车子的80%乙醇提取物（80ET）根据其极性分离为二氯甲烷（DCM）和水相部分（WF），并研究了80ET、DCM和WF的抗增殖作用。小檗碱是箭叶风车子的主要成分之一，用作对照。通过细胞生长试验评估，80ET、DCM、WF和小檗碱均显示出抗增殖活性。随后，提取物及其各部分使促凋亡蛋白NAG-1和ATF3增加，细胞周期蛋白细胞周期蛋白D1减少。有趣的是，只有DCM部分表现出过氧化物酶体增殖物激活受体γ（PPARγ）结合活性的诱导，这在结肠癌细胞中代表一种促凋亡活性。本研究的总体结果表明，该植物提取物通过激活促凋亡蛋白和PPARγ具有抗增殖活性，可能具有作为癌症治疗预防方案的潜力。
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