Liu Yali, Zhou Mingyue, Jin Chen, Zeng Jinxiang, Huang Chao, Song Qiuye, Song Yonggui
Laboratory Animal Science and Technology Center, College of Science and Technology, Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
Pharmacy Department of Zhangjiagang, First People's Hospital, Suzhou, China.
Front Chem. 2020 Aug 28;8:643. doi: 10.3389/fchem.2020.00643. eCollection 2020.
In this work, a covalent-organic framework with high carbon and nitrogen content microstructures (named COF-LZU1), assisted by 3D nitrogen-containing kenaf stem composites (represented as COF-LZU1/3D-KSCs), was constructed. Moreover, it was utilized for immobilizing acetylcholinesterase (AChE) for identifying trichlorfon, a commonly applied organophosphorus (OP) pesticide. The development of COF-LZU1/3D-KSC was affirmed by SEM, PXRD, and EDXS. The findings confirmed that COF-LZU1 microstructures were uniformly developed on 3D-KSC holes using a one-step synthesis approach, which can substantially enhance the effective surface area. Also, the COF-LZU1/3D-KSC composite contains not only the nitrogen element in COF-LZU1 but also the nitrogen element in 3D-KSC, which will greatly improve the biocompatibility of the material. The AChE/COF-LZU1/3D-KSC integrated electrode was fabricated by directly fixing a large amount of AChE on the composite. At the same time, the integrated electrode had good detection efficiency for trichlorfon. Improved stabilization, a wide-linear-range (0.2-19 ng/mL), and a lower detection limit (0.067 ng/mL) have been displayed by the sensor. Therefore, this sensor can be used as an important platform for the on-site detection of OP residue.
在这项工作中，构建了一种具有高碳氮含量微观结构的共价有机框架（命名为COF-LZU1），并以三维含氮红麻茎复合材料（表示为COF-LZU1/3D-KSCs）为辅助。此外，它被用于固定乙酰胆碱酯酶（AChE）以识别敌百虫，一种常用的有机磷（OP）农药。通过扫描电子显微镜（SEM）、粉末X射线衍射（PXRD）和能量散射X射线光谱（EDXS）证实了COF-LZU1/3D-KSC的形成。研究结果证实，采用一步合成法，COF-LZU1微观结构在3D-KSC孔上均匀生长，这可大幅提高有效表面积。此外，COF-LZU1/3D-KSC复合材料不仅含有COF-LZU1中的氮元素，还含有3D-KSC中的氮元素，这将大大提高材料的生物相容性。通过将大量AChE直接固定在复合材料上制备了AChE/COF-LZU1/3D-KSC集成电极。同时，该集成电极对敌百虫具有良好的检测效率。该传感器表现出更好的稳定性、宽线性范围（0.2 - 19 ng/mL）和较低的检测限（0.067 ng/mL）。因此，该传感器可作为现场检测OP残留的重要平台。
