Lin Xiaofeng, Mei Yanqiu, He Chen, Luo Yan, Yang Min, Kuang Ying, Ma Xiaoming, Zhang Huifang, Huang Qitong
Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education, Key Laboratory of Biomaterials and Biofabrication in Tissue Engineering of Jiangxi Province, Oil-tea in Medical Health Care and Functional Product Development Engineering Research Center in Jiangxi, School of Public Health and Health Management, School of Pharmacy, School of Medical and Information Engineering, The Science Research Center, Gannan Medical University, Ganzhou, China.
School of Chemistry and Chemical Engineering, Key Laboratory of Organo-pharmaceutical Chemistry of Jiangxi Province, Gannan Normal University, Ganzhou, China.
Front Chem. 2021 Nov 19;9:769648. doi: 10.3389/fchem.2021.769648. eCollection 2021.
() O157:H7 can cause many food safety incidents, which seriously affect human health and economic development. Therefore, the sensitive, accurate, and rapid determination of O157:H7 is of great significance for preventing the outbreak and spread of foodborne diseases. In this study, a carbon dots-FeO nanomaterial (CDs-FeO)-based sensitive electrochemical biosensor for O157:H7 detection was developed. The CDs have good electrical conductivity, and the surface of carbon dots contains abundant carboxyl groups, which can be used to immobilize probe DNA. Meanwhile, the CDs can be used as a reducing agent to prepare CDs-FeO nanomaterial. The FeO nanomaterial can improve the performance of the electrochemical biosensor; it also can realize the recovery of CDs-FeO due to its magnetism. As expected, the electrochemical biosensor has excellent specificity of O157:H7 among other bacteria. The electrochemical biosensor also exhibited good performance for detecting O157:H7 with the detection range of 10-10 CFU/ml, and the detection limit of this electrochemical biosensor was 6.88 CFU/ml (3S/N). Furthermore, this electrochemical biosensor was successfully used for monitoring O157:H7 in milk and water samples, indicating that this electrochemical biosensor has good application prospect. More importantly, this research can provide a new idea for the detection of other bacteria and viruses.
O157:H7可引发诸多食品安全事件，严重影响人类健康和经济发展。因此，灵敏、准确且快速地检测O157:H7对于预防食源性疾病的爆发和传播具有重要意义。在本研究中，开发了一种基于碳点 - 氧化亚铁纳米材料（CDs - FeO）的用于检测O157:H7的灵敏电化学生物传感器。碳点具有良好的导电性，其表面含有丰富的羧基，可用于固定探针DNA。同时，碳点可作为还原剂来制备CDs - FeO纳米材料。氧化亚铁纳米材料可提高电化学生物传感器的性能；因其具有磁性，还可实现CDs - FeO的回收。正如预期的那样，该电化学生物传感器在其他细菌中对O157:H7具有优异的特异性。该电化学生物传感器在检测O157:H7时也表现出良好的性能，检测范围为10⁻¹⁰ CFU/ml，该电化学生物传感器的检测限为6.88 CFU/ml（3S/N）。此外，该电化学生物传感器成功用于监测牛奶和水样中的O157:H7，表明该电化学生物传感器具有良好的应用前景。更重要的是，本研究可为其他细菌和病毒的检测提供新思路。
