Horiuk Yulia, Horiuk Victor, Kukhtyn Mykola, Tsvihun Anatoliy, Kernychnyi Sergiy
Faculty of Veterinary Medicine and Technologies in Livestock, Department of Infectious and Parasitic Diseases, State Agrarian and Engineering University in Podilya, Kamianets-Podilskyi, Ukraine.
Faculty of Veterinary Medicine and Technologies in Livestock, Department of Veterinary Obstetrics, Pathology and Surgery, State Agrarian and Engineering University in Podilya, Kamianets-Podilskyi, Ukraine.
J Adv Vet Anim Res. 2020 Aug 22;7(3):509-513. doi: 10.5455/javar.2020.g447. eCollection 2020 Sep.
The objective of this study was to investigate the intensity of phage infection caused by , which was isolated from dairy farms, depending on the initial number of cells in the medium.
To evaluate the impact of the viable bacteria on the intensity of phage infection caused by , 1 mg of phagolysate (phage titer 10 CFU/ ml) was introduced in 9 ml of nutrient broth with an appropriate amount of daily culture of under study. The number of viable staphylococci was determined by total viable count/ml.
In this experiment, we found that the intensity of phages lytic activity was dependent on the number of sensitive bacterial cells in the volume of the culture medium. Effective phage therapy requires a high concentration of phages in the medium (inflammation foci) for rapid contact of the virus with bacteria.
When developing a phage drug to treat subclinical mastitis, it is necessary to increase the phage titer in the drug or its dosage compared to the clinical form, as there is a lower probability of phage contact with a susceptible microbial cell. Besides, at a high concentration of bacteria, there is a gradual decrease in nutrients in the medium, resulting in phages going back to the condition of lysogeny.
本研究的目的是调查从奶牛场分离出的噬菌体感染的强度，该强度取决于培养基中初始细胞的数量。
为评估活细菌对噬菌体感染强度的影响，将1毫克噬菌溶胞产物（噬菌体滴度为10CFU/毫升）引入9毫升营养肉汤中，并加入适量正在研究的每日培养物。通过每毫升总活菌数来确定活葡萄球菌的数量。
在本实验中，我们发现噬菌体裂解活性的强度取决于培养基体积中敏感细菌细胞的数量。有效的噬菌体疗法需要培养基（炎症病灶）中有高浓度的噬菌体，以便病毒与细菌快速接触。
在开发用于治疗亚临床乳腺炎的噬菌体药物时，与临床形式相比，有必要提高药物中的噬菌体滴度或其剂量，因为噬菌体与易感微生物细胞接触的可能性较低。此外，在细菌浓度高时，培养基中的营养物质会逐渐减少，导致噬菌体回到溶原状态。
