Univ. Polytechnique Hauts-de-France, EA 2443 - LMCPA - Laboratoire des Matériaux Céramiques et Procédés Associés, F-59313 Valenciennes, France; Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.
Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.
Mater Sci Eng C Mater Biol Appl. 2020 Jun;111:110840. doi: 10.1016/j.msec.2020.110840. Epub 2020 Mar 12.
This study provides a new therapeutic response to postoperative joint and bone infections. Alone or in combination with antibiotics, phage therapy has many advantages, including accurate targeting of pathogenic bacteria. In addition, a decrease in harmful side effects can improve the healing process. Integrating the bacteriophage directly into the graft product will improve the antibacterial spread over the site of the surgery. The phage cocktail-filled ceramics are an innovative device for localized and curative phage therapy (in prosthetic replacement surgery, for example) in bone and joint surgery. Calcium phosphate-based ceramics were synthesized and shaped by stereolithography (3D) before loading by a phage cocktail to lyse a heterospecific bacterial population. In addition, the device makes possible the protection of osteoblastic cells against Staphylococcus aureus infection during their colonization on the ceramic material and prevents the formation of biofilm on the surface of biomaterials.
本研究为术后关节和骨骼感染提供了一种新的治疗反应。噬菌体疗法单独或与抗生素联合使用具有许多优势，包括准确靶向病原菌。此外，减少有害的副作用可以改善愈合过程。将噬菌体直接整合到移植物产品中，将提高手术部位的抗菌扩散效果。噬菌体鸡尾酒填充陶瓷是一种用于骨与关节手术中局部和治疗性噬菌体治疗（例如在假体置换手术中）的创新装置。通过立体光刻（3D）合成并成型磷酸钙基陶瓷，然后用噬菌体鸡尾酒加载以裂解异源细菌种群。此外，该装置可防止生物膜在生物材料表面形成，保护成骨细胞免受定植在陶瓷材料上的金黄色葡萄球菌感染。
