Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea.
Arch Virol. 2020 Dec;165(12):2927-2930. doi: 10.1007/s00705-020-04827-9. Epub 2020 Oct 1.
This study describes the first isolation and genetic characterization of the newly emerging porcine circovirus type 2e (PCV2e) from Korean pigs. The PCV2e isolate did not produce a cytopathic effect in PCV-free PK-15 cells; therefore, PCV2e infection was demonstrated by immunohistochemistry with polyclonal PCV2a antibodies and polymerase chain reaction with primers specific for PCV2e. As the infected PCV-free PK-15 cells were passaged, the amount of infectious virus correlated with an increase in the amount of viral DNA (i.e., a decrease in the cycle threshold values. A full genomic analysis of the PCV2e strain SNUVR199711 was performed and showed that the genome is 1,777 nucleotides in length.
本研究描述了首例从韩国猪中分离到的新型猪圆环病毒 2 型（PCV2e）及其遗传特征。该 PCV2e 分离株在无 PCV 的 PK-15 细胞中不会产生细胞病变效应；因此，通过针对 PCV2a 的多克隆抗体进行免疫组织化学染色和针对 PCV2e 的引物进行聚合酶链反应证实了 PCV2e 感染。随着感染无 PCV 的 PK-15 细胞的传代，感染性病毒的量与病毒 DNA 量的增加相关（即循环阈值的降低。对 PCV2e 株 SNUVR199711 的全基因组分析表明，该基因组全长 1777 个核苷酸。
