Australian Research Council Training Centre for Biopharmaceutical Innovation, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Corner College and Cooper Roads, Brisbane, Queensland, 4072, Australia.
Protein Expression Facility, The University of Queensland, Corner College and Cooper Roads, Brisbane, Queensland, 4072, Australia.
Biotechnol J. 2021 Mar;16(3):e2000309. doi: 10.1002/biot.202000309. Epub 2020 Oct 19.
The need to intensify downstream processing of monoclonal antibodies to complement the advances in upstream productivity has led to increased attention toward implementing membrane technologies. With the industry moving toward continuous operations and single use processes, membrane technologies show promise in fulfilling the industry needs due to their operational flexibility and ease of implementation. Recently, the applicability of membrane-based unit operations in integrating the downstream process has been explored. In this article, the major developments in the application of membrane-based technologies in the bioprocessing of monoclonal antibodies are reviewed. The recent progress toward developing intensified end-to-end bioprocesses and the critical role membrane technology will play in achieving this goal are focused upon.
需要加强单克隆抗体的下游处理，以补充上游生产力的提高，这导致人们越来越关注膜技术的应用。随着行业向连续操作和一次性使用过程的发展，膜技术由于其操作灵活性和易于实施，有望满足行业需求。最近，已经探索了基于膜的单元操作在整合下游工艺中的应用。本文综述了膜基技术在单克隆抗体生物加工中的主要应用进展。重点介绍了开发强化端到端生物工艺的最新进展，以及膜技术在实现这一目标中所起的关键作用。
