Anosov A A, Smirnova E Yu, Ryleeva E D, Gligonov I A, Korepanova E A, Sharakshane A A
I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia.
Kotelnikov Institute of Radioengineering and Electronics of RAS, Moscow, Russia.
Eur Phys J E Soft Matter. 2020 Oct 6;43(10):66. doi: 10.1140/epje/i2020-11989-0.
The conductive lipid pores occurring in planar bilayer membranes are known to manifest themselves experimentally as current fluctuations. Reliable recording of such fluctuations during phase transitions, as well as in membranes with various additives (for example, SDS), allows one to determine the characteristics of hypothetical hydrophilic pores, namely, their number, sizes, lifetimes, and duration of time intervals between pores. Because, in contrast with electroporation, the emergence of pores in a membrane does not require high voltages, this process is called soft poration. Studying the characteristics of pores under soft poration allows us to estimate the parameters of the Smoluchowski equation and compare them with the corresponding parameters used to describe electroporation. In this work, the experimental characteristics of current fluctuations in the membrane with the addition of SDS to the bulk solution were used to estimate the parameters of the Smoluchowski equation: the pore edge tension, the energy of the hydrophobic pore/hydrophilic pore barrier, the coefficient of pore diffusion in the radius space, the initial distribution density of the number of pores, and the attempt rate density of the lipids in a membrane. The obtained estimates are close to the parameter values used in studies of electroporation.
已知在平面双层膜中出现的导电脂质孔在实验中表现为电流波动。在相变过程中以及在含有各种添加剂（例如十二烷基硫酸钠）的膜中可靠地记录此类波动，能够确定假设的亲水性孔的特征，即它们的数量、大小、寿命以及孔之间的时间间隔持续时间。因为与电穿孔不同，膜中孔的出现不需要高电压，所以这个过程被称为软打孔。研究软打孔下孔的特征使我们能够估计斯莫卢霍夫斯基方程的参数，并将它们与用于描述电穿孔的相应参数进行比较。在这项工作中，利用向本体溶液中添加十二烷基硫酸钠的膜中电流波动的实验特征来估计斯莫卢霍夫斯基方程的参数：孔边缘张力、疏水孔/亲水孔屏障的能量、孔在半径空间中的扩散系数、孔数量的初始分布密度以及膜中脂质的尝试速率密度。所获得的估计值与电穿孔研究中使用的参数值相近。
