Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, 350122, China; Department of Geriatric Medicine, the Second Affiliated Hospital of Fujian Traditional Chinese Medical University, Fuzhou, Fujian, 350003, China; Fujian Key Laboratory of Geriatrics, Fuzhou, Fujian, 350001, China; Fujian Provincial Center for Geriatrics, Fuzhou, Fujian, 350001, China.
Fujian Key Laboratory of Geriatrics, Fuzhou, Fujian, 350001, China; Fujian Provincial Center for Geriatrics, Fuzhou, Fujian, 350001, China; Department of Geriatric Medicine, Fujian Provincial Hospital, Fuzhou, Fujian, 350001, China.
Eur J Pharmacol. 2020 Dec 15;889:173610. doi: 10.1016/j.ejphar.2020.173610. Epub 2020 Sep 29.
Salvianolate is a compound from traditional Chinese medicine widely used in the treatment of various cardiovascular diseases. This study explored the effects of salvianolate on myocardial infarction and used tandem mass tags (TMT) to discover differentially expressed proteins. Male Sprague Dawley rats were randomly divided into the sham operation group, model group, and salvianolate group. The myocardial infarction model was established by ligating the left anterior descending coronary artery while the sham group had a sham operation. The rats were intraperitoneally injected with 2 ml of 5% glucose once a day, with 48.438 mg/kg/d salvianolate for the rats in the salvianolate group. After 4 weeks, the rats' hemodynamics were measured to evaluate cardiac function, and Masson staining assessed the area of myocardial infarction. TMT analysis was performed and validated by western blot. Salvianolate improved cardiac function after myocardial infarction, reduced the myocardial infarction area, and protected the myocardial tissue. 100 differentially expressed proteins were identified between the sham operation and model groups, salvianolate reversed the expression of 25 of those proteins, that were mainly involved in the metabolism of extracellular collagen matrix and the response to growth factor stimulation. Type I collagen, type V collagen, chymase, β-myosin heavy chain, and A-Raf differential expression were consistent in western blotting. In conclusion, salvianolate had a protective effect on myocardial tissues of rats with myocardial infarction. Several proteins including type I collagen, type V collagen, chymase, β-myosin, and A-Raf may be salvianolate targets for treatment of myocardial infarction.
丹酚酸 B 是一种来源于中药的化合物，广泛用于治疗各种心血管疾病。本研究探讨了丹酚酸 B 对心肌梗死的作用，并使用串联质量标签（TMT）发现差异表达蛋白。雄性 Sprague Dawley 大鼠随机分为假手术组、模型组和丹酚酸 B 组。结扎左前降支冠状动脉建立心肌梗死模型，假手术组进行假手术。丹酚酸 B 组大鼠每天腹腔注射 5%葡萄糖溶液 2ml，剂量为 48.438mg/kg/d。4 周后，测量大鼠血流动力学以评估心功能，Masson 染色评估心肌梗死面积。通过 Western blot 验证 TMT 分析。丹酚酸 B 改善心肌梗死后的心功能，减少心肌梗死面积，保护心肌组织。假手术组和模型组之间鉴定出 100 种差异表达蛋白，丹酚酸 B 逆转了其中 25 种蛋白的表达，这些蛋白主要参与细胞外胶原基质代谢和生长因子刺激反应。I 型胶原、V 型胶原、糜酶、β-肌球蛋白重链和 A-Raf 的差异表达在 Western blot 中一致。总之，丹酚酸 B 对心肌梗死后大鼠的心肌组织有保护作用。几种蛋白，包括 I 型胶原、V 型胶原、糜酶、β-肌球蛋白和 A-Raf，可能是丹酚酸 B 治疗心肌梗死的靶点。
