Rollins-Smith Louise A, Smith Patricia B, Ledeczi Anna M, Rowe Julia M, Reinert Laura K
Departments of Pathology, Microbiology and Immunology and of Pediatrics, Vanderbilt University School of Medicine and Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232, USA.
Antibiotics (Basel). 2020 Sep 30;9(10):661. doi: 10.3390/antibiotics9100661.
Although acquired immunodeficiency syndrome (AIDS) caused by the human immunodeficiency virus (HIV) is a manageable disease for many, it is still a source of significant morbidity and economic hardship for many others. The predominant mode of transmission of HIV/AIDS is sexual intercourse, and measures to reduce transmission are needed. Previously, we showed that caerin 1 antimicrobial peptides (AMPs) originally derived from Australian amphibians inhibited in vitro transmission of HIV at relatively low concentrations and had low toxicity for T cells and an endocervical cell line. The use of AMPs as part of microbicidal formulations would expose the vaginal microbiome to these agents and cause potential harm to protective lactobacilli. Here, we tested the effects of caerin 1 peptides and their analogs on the viability of two species of common vaginal lactobacilli ( and ). Several candidate peptides had limited toxicity for the lactobacilli at a range of concentrations that would inhibit HIV. Three AMPs were also tested for their ability to inhibit growth of a close relative of the sexually transmissible was significantly more sensitive to the AMPs than the lactobacilli. Thus, several candidate AMPs have the capacity to inhibit HIV and possible transmission at concentrations that are significantly less harmful to the resident lactobacilli.
尽管人类免疫缺陷病毒（HIV）引起的获得性免疫缺陷综合征（AIDS）对许多人来说是一种可控制的疾病，但对其他许多人而言，它仍是严重发病和经济困难的根源。HIV/AIDS的主要传播方式是性交，因此需要采取措施减少传播。此前，我们发现最初源自澳大利亚两栖动物的凯林1抗菌肽（AMPs）在相对较低浓度下可抑制HIV的体外传播，并且对T细胞和子宫颈细胞系毒性较低。将AMPs用作杀微生物制剂的一部分会使阴道微生物群接触这些制剂，并对保护性乳酸杆菌造成潜在损害。在此，我们测试了凯林1肽及其类似物对两种常见阴道乳酸杆菌（ 和 ）活力的影响。在一系列可抑制HIV的浓度范围内，几种候选肽对乳酸杆菌的毒性有限。我们还测试了三种AMPs抑制 生长的能力， 是性传播 的近亲，它对AMPs的敏感性明显高于乳酸杆菌。因此，几种候选AMPs有能力在对常驻乳酸杆菌危害明显较小的浓度下抑制HIV及可能的 传播。
