Department of Food and Environmental Virology, National Veterinary Research Institute, Al. Partyzantów 57, 24-100 Puławy, Poland.
Department of Large Animal Diseases and Clinic, Warsaw University of Life Sciences, Nowoursynowska Street 100, 02-797 Warsaw, Poland.
Viruses. 2020 Sep 30;12(10):1113. doi: 10.3390/v12101113.
The most important wildlife species in the epidemiology of hepatitis E virus (HEV) infections are wild boars, which are also the main reservoir of the virus in a sylvatic environment. The aim of the study was a serological and molecular assessment of the prevalence of HEV infections in wild boars in Poland. In total, 470 pairs of samples (wild boar blood and livers) and 433 samples of faeces were tested. An ELISA (ID.vet, France) was used for serological analysis. For the detection of HEV RNA, real-time (RT)-qPCR was employed. The presence of specific anti-HEV IgG antibodies was found in 232 (49.4%; 95%CI: 44.7-54%) sera, with regional differences observed in the seroprevalence of infections. HEV RNA was detected in 57 (12.1%, 95%CI: 9.3-15.4%) livers and in 27 (6.2%, 95%CI: 4.1-8.9%) faecal samples, with the viral load ranging from 1.4 to 1.7 × 10 G.C./g and 38 to 9.3 × 10 G.C./mL, respectively. A correlation between serological and molecular results of testing of wild boars infected with HEV was shown. HEV infections in wild boars appeared to be common in Poland.
在戊型肝炎病毒 (HEV) 感染的流行病学中，最重要的野生动物物种是野猪，它们也是森林环境中病毒的主要宿主。本研究的目的是对波兰野猪中 HEV 感染的流行情况进行血清学和分子评估。共检测了 470 对（野猪血液和肝脏）样本和 433 份粪便样本。采用 ELISA（ID.vet，法国）进行血清学分析。为了检测 HEV RNA，采用了实时（RT）-qPCR。在 232 份（49.4%；95%CI：44.7-54%）血清中发现了特异性抗-HEV IgG 抗体，感染的血清流行率存在地区差异。在 57 份（12.1%；95%CI：9.3-15.4%）肝脏和 27 份（6.2%；95%CI：4.1-8.9%）粪便样本中检测到 HEV RNA，病毒载量范围分别为 1.4 至 1.7×10 G.C./g 和 38 至 9.3×10 G.C./mL。显示了感染 HEV 的野猪的血清学和分子检测结果之间的相关性。HEV 感染在波兰的野猪中似乎很常见。
