从安大略省屠宰牛的肾脏中分离出哈氏钩端螺旋体。
Isolation of Leptospira hardjo from kidneys of Ontario cattle at slaughter.
作者信息
Prescott J F, Miller R B, Nicholson V M
出版信息
Can J Vet Res. 1987 Apr;51(2):229-31.
Kidneys from 117 cattle from 110 Ontario farms were examined at slaughter for leptospires. Leptospira hardjo (hardjo-bovis A) was isolated from 11 kidneys and L. kennewicki from one. The isolations were all made (12/89, 13.5%) from beef cattle from feedlots, no isolates being obtained from dairy or beef cattle from extensive farms (0/28). Isolations were only made from cattle with antibody titers (greater than or equal to 20) against the serovars recovered. Isolation was more sensitive than immunofluorescence in identifying leptospira, particularly in animals with low antibody titers against L. hardjo. Leptospira were isolated from two kidneys with multiple gross lesions of focal nephritis, but there was no correlation between the presence of scanty kidney lesions and isolations of leptospira. Leptospira hardjo infection appears to be common in Ontario feedlot cattle.
对来自安大略省110个农场的117头牛的肾脏在屠宰时进行了钩端螺旋体检查。从11个肾脏中分离出了哈氏钩端螺旋体（哈氏牛型A），从1个肾脏中分离出了肯氏钩端螺旋体。所有分离株均来自饲养场的肉牛（12/89，13.5%），未从粗放式农场的奶牛或肉牛中获得分离株（0/28）。仅从对所分离血清型具有抗体效价（大于或等于20）的牛中分离出病原体。在鉴定钩端螺旋体方面，分离培养比免疫荧光更敏感，尤其是在对哈氏钩端螺旋体抗体效价较低的动物中。从两个有局灶性肾炎多处肉眼病变的肾脏中分离出了钩端螺旋体，但肾脏轻微病变的存在与钩端螺旋体的分离之间没有相关性。哈氏钩端螺旋体感染在安大略省饲养场的牛中似乎很常见。
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