Kitson-Piggot A W, Prescott J F
Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph.
Can J Vet Res. 1987 Oct;51(4):448-51.
Sera from Thoroughbred and Standardbred horses in southwest Ontario were tested for antibody to seven Leptospira interrogans serovars (autumnalis, bratislava, canicola, grippotyphosa, hardjo, icterohaemorrhagiae, pomona), using the microscopic agglutination test. There was significantly higher seroprevalence of bratislava than of other serovars, in which prevalence was low. Seroprevalence of bratislava increased significantly with age; only 5% of two to three year old horses had titers greater than or equal to 1:80 compared to 52% of horses older than seven years. Eight of 16 foals from two farms seroconverted at low titers to bratislava between four and eight months of age. Leptospires were not detected by immunofluorescence and isolation techniques in 50 kidneys collected from horses at slaughter. Fetal tissues from 52 aborted horse fetuses were also examined by these methods and serovar kennewicki was identified by immunofluorescence and by isolation in one fetus. Serovar bratislava appears to be widespread in horses in Ontario but unimportant in abortion. The clinical significance of this infection in horses in Ontario is unclear.
采用显微镜凝集试验，对安大略省西南部纯种马和标准赛马的血清进行了七种问号钩端螺旋体血清型（秋季热、布拉迪斯拉发、犬型、爪哇热、哈焦、出血性黄疸、波摩那）抗体检测。布拉迪斯拉发血清型的血清阳性率显著高于其他血清型，其他血清型的阳性率较低。布拉迪斯拉发血清型的血清阳性率随年龄显著增加；两到三岁的马中只有5%的效价大于或等于1:80，而七岁以上的马中这一比例为52%。来自两个农场的16匹幼驹中有8匹在4至8个月大时血清转化为低滴度的布拉迪斯拉发血清型。在屠宰时收集的50个马肾脏中，通过免疫荧光和分离技术未检测到钩端螺旋体。还通过这些方法对52例流产马胎儿的胎儿组织进行了检查，通过免疫荧光和在一个胎儿中分离鉴定出肯尼威克血清型。布拉迪斯拉发血清型似乎在安大略省的马匹中广泛存在，但在流产中并不重要。这种感染在安大略省马匹中的临床意义尚不清楚。
