College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, Jilin, China.
College of Life Science, Changchun Sci-Tech University, Changchun, Jilin, China.
Ann N Y Acad Sci. 2021 Feb;1486(1):58-75. doi: 10.1111/nyas.14480. Epub 2020 Oct 2.
Aeromonas veronii is an important zoonotic and aquatic pathogen. An increasing number of reports indicate that it has caused substantial economic losses in the aquaculture industry, in addition to threatening human health. However, little is known about its pathogenesis. Exploration of new virulence factors of A. veronii would be helpful for further understanding its pathogenesis. Hence, we comparatively analyzed the proteomes of virulent, attenuated, and avirulent strains of A. veronii using tandem mass tag (TMT) protein labeling and found numerous proteins either up- or downregulated in the virulent strain. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses showed that these differentially expressed proteins (DEPs) were involved mainly in pathways associated with bacterial chemotaxis and microbial metabolism in diverse environments. Furthermore, the expression levels of lysine decarboxylase, endoribonuclease, maltoporin, pullulanase, and aerolysin were positively correlated with the virulence of the strains, suggesting that their function may be closely related to the virulence of A. veronii. The results of qRT-PCR and multiple reaction monitoring for some DEPs were consistent with the results of TMT protein labeling. These results suggest that these DEPs may be novel potential virulence factors and will help to further understand the pathogenesis of A. veronii.
维氏气单胞菌是一种重要的人畜共患病和水生病原体。越来越多的报告表明，它除了威胁人类健康外，还对水产养殖业造成了巨大的经济损失。然而，人们对其发病机制知之甚少。探索维氏气单胞菌的新毒力因子将有助于进一步了解其发病机制。因此，我们使用串联质量标签（TMT）蛋白标记法比较分析了强毒、减毒和无毒菌株的蛋白质组，发现强毒菌株中存在大量上调或下调的蛋白质。基因本体论和京都基因与基因组百科全书通路分析表明，这些差异表达蛋白（DEPs）主要参与与细菌趋化性和微生物代谢相关的通路，这些通路存在于各种环境中。此外，赖氨酸脱羧酶、内切核酸酶、麦芽寡糖素、普鲁兰酶和 aerolysin 的表达水平与菌株的毒力呈正相关，这表明它们的功能可能与维氏气单胞菌的毒力密切相关。一些 DEPs 的 qRT-PCR 和多重反应监测结果与 TMT 蛋白标记的结果一致。这些结果表明，这些 DEPs 可能是新的潜在毒力因子，将有助于进一步了解维氏气单胞菌的发病机制。
