Laboratory of Experimental Medicine (ULB222), Faculty of Medicine, Université libre de Bruxelles, CHU de Charleroi, Charleroi, Belgium.
Computer Science Department, University of Geneva, Geneva, Switzerland.
Ann N Y Acad Sci. 2021 Feb;1485(1):71-82. doi: 10.1111/nyas.14489. Epub 2020 Oct 2.
Red blood cells (RBCs) in pathological situations undergo biochemical and conformational changes, leading to alterations in rheology involved in cardiovascular events. The shape of RBCs in volunteers and stable and exacerbated chronic obstructive pulmonary disease (COPD) patients was analyzed. The effects of RBC spherization on platelet transport (displacement in the flow field caused by their interaction with RBCs) were studied in vitro and by numerical simulations. RBC spherization was observed in COPD patients compared with volunteers. In in vitro experiments at a shear rate of 100 s , treatment of RBCs with neuraminidase induced greater sphericity, which mainly affected platelet aggregates without changing aggregate size. At 400 s , neuraminidase treatment changes both the size of the aggregates and the number of platelet aggregates. Numerical simulations indicated that RBC spherization induces an increase of the platelet mean square displacement, which is traditionally linked to the platelet diffusion coefficient. RBCs of COPD patients are more spherical than healthy volunteers. Experimentally, RBC spherization induces increased platelet transport to the wall. Additional studies are needed to understand the link between the effect of RBCs on platelet transport and the increased cardiovascular events observed in COPD patients.
在病理情况下，红细胞（RBC）经历生化和构象变化，导致涉及心血管事件的流变学改变。分析了志愿者和稳定及加重的慢性阻塞性肺疾病（COPD）患者的红细胞形状。研究了 RBC 变形对血小板运输（由于与 RBC 的相互作用在流场中引起的位移）的体外和数值模拟影响。与志愿者相比，在 COPD 患者中观察到 RBC 变形。在剪切率为 100 s 的体外实验中，用唾液酸酶处理 RBC 诱导更大的变形性，这主要影响血小板聚集而不改变聚集大小。在 400 s 时，唾液酸酶处理改变了聚集物的大小和血小板聚集物的数量。数值模拟表明，RBC 变形会导致血小板均方根位移增加，这与血小板扩散系数传统上相关。COPD 患者的 RBC 比健康志愿者更圆。实验表明，RBC 变形会增加血小板向壁面的运输。需要进一步研究以了解 RBC 对血小板运输的影响与 COPD 患者观察到的心血管事件增加之间的联系。
