Lee Hyunjin, Jung Dong-Hyun, Seo Dong-Ho, Chung Won-Hyong, Seo Myung-Ji
Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University, Incheon 22012, Republic of Korea.
Bacteria Research Team, Nakdonggang National Institute of Biological Resources, Sangju 37242, Republic of Korea.
Genomics. 2021 Jan;113(1 Pt 2):647-653. doi: 10.1016/j.ygeno.2020.09.061. Epub 2020 Oct 1.
1-Deoxynojirumycin (1-DNJ) is a representative iminosugar with α-glucosidase inhibition (AGI) activity. In this study, the full genome sequencing of 1-DNJ-producing Bacillus velezensis K26 was performed. The genome consists of a circular chromosome (4,047,350 bps) with two types of putative virulence factors, five antibiotic resistance genes, and seven secondary metabolite biosynthetic gene clusters. Genomic analysis of a wide range of Bacillus species revealed that a 1-DNJ biosynthetic gene cluster was commonly present in four Bacillus species (B. velezensis, B. pseudomycoides, B. amyloliquefaciens, and B. atrophaeus). In vitro experiments revealed that the increased mRNA expression levels of the three 1-DNJ biosynthetic genes were closely related to increased AGI activity. Genomic comparison and alignment of multiple gene sequences indicated the conservation of the 1-DNJ biosynthetic gene cluster in each Bacillus species. This genomic analysis of Bacillus species having a 1-DNJ biosynthetic gene cluster could provide a basis for further research on 1-DNJ-producing bacteria.
1-脱氧野尻霉素（1-DNJ）是一种具有α-葡萄糖苷酶抑制（AGI）活性的代表性亚氨基糖。在本研究中，对产1-DNJ的贝莱斯芽孢杆菌K26进行了全基因组测序。该基因组由一个环状染色体（4,047,350 bp）组成，含有两种假定的毒力因子、五个抗生素抗性基因和七个次级代谢产物生物合成基因簇。对多种芽孢杆菌属物种的基因组分析表明，一个1-DNJ生物合成基因簇普遍存在于四种芽孢杆菌属物种（贝莱斯芽孢杆菌、假霉菌芽孢杆菌、解淀粉芽孢杆菌和萎缩芽孢杆菌）中。体外实验表明，三个1-DNJ生物合成基因的mRNA表达水平升高与AGI活性增加密切相关。多个基因序列的基因组比较和比对表明，1-DNJ生物合成基因簇在每个芽孢杆菌属物种中具有保守性。对具有1-DNJ生物合成基因簇的芽孢杆菌属物种的这种基因组分析可为进一步研究产1-DNJ的细菌提供基础。
