Siziya Inonge Noni, Lim Hyo Jung, Jung Dong-Hyun, Baek Suhyeon, Lee Sanggil, Seo Myung-Ji
Division of Bioengineering, Incheon National University, Incheon, 22012 Republic of Korea.
Research Center for Bio Materials & Process Development, Incheon National University, Incheon, 22012 Republic of Korea.
Food Sci Biotechnol. 2025 Feb 15;34(10):2225-2235. doi: 10.1007/s10068-025-01834-x. eCollection 2025 Jun.
The iminosugars 1-deoxynojirimycin (1-DNJ) and 1-deoxymannojirimycin (1-DMJ) were produced by recombinant (TYB) bearing the 1-DNJ-producing GabT1-Yktc1-GutB1 (TYB) gene cluster from MBLB0692. The enhanced iminosugar biosynthesis in TYB cultures increased both α-mannosidase inhibition (AMI) and α-glucosidase inhibition (AGI) activity. Individual cultures harboring GabT1, Yktc1, and GutB1 genes were found to be able to produce both 1-DNJ and 1-DMJ in mixed cultures of one pot analysis. This suggests that the genes necessary for epimerization and reduction are intrinsic to the cells rather than close to the 1-DNJ-producing cluster. Glucose was the preferred carbon source and a provision of 10 g/L glucose increased AGI and AMI to 69.8% and 70.1%, respectively. The 1 mM IPTG produced 55.0% AGI and 72.1% AMI, and 20% ethanolic permeabilization produced 62.8% AGI and 74.2% AMI. Batch fermentation increased iminosugar yields from 18.7 to 229.9 mg/L 1-DNJ, and 41.8 to 63.7 mg/mL 1-DMJ.
The online version contains supplementary material available at 10.1007/s10068-025-01834-x.
亚氨基糖1-脱氧野尻霉素（1-DNJ）和1-脱氧甘露野尻霉素（1-DMJ）由携带来自MBLB0692的产1-DNJ的GabT1-Yktc1-GutB1（TYB）基因簇的重组体（TYB）产生。TYB培养物中亚氨基糖生物合成的增强增加了α-甘露糖苷酶抑制（AMI）和α-葡萄糖苷酶抑制（AGI）活性。发现在单罐分析的混合培养物中，携带GabT1、Yktc1和GutB1基因的单个培养物能够产生1-DNJ和1-DMJ。这表明差向异构化和还原所需的基因是细胞固有的，而不是靠近产1-DNJ的簇。葡萄糖是首选碳源，提供10 g/L葡萄糖可分别将AGI和AMI提高到69.8%和70.1%。1 mM异丙基-β-D-硫代半乳糖苷（IPTG）产生55.0%的AGI和72.1%的AMI，20%乙醇透化产生62.8%的AGI和74.2%的AMI。分批发酵使亚氨基糖产量从18.7 mg/L的1-DNJ增加到229.9 mg/L，从41.8 mg/mL的1-DMJ增加到63.7 mg/mL。
在线版本包含可在10.1007/s10068-025-01834-x获取的补充材料。
