Department of Endodontics, Faculty of Dentistry, Bolu Abant Izzet Baysal University Bolu, Turkey.
Department of Endodontics, Faculty of Dentistry, Bolu Abant Izzet Baysal University Bolu, Turkey.
Photodiagnosis Photodyn Ther. 2020 Dec;32:102038. doi: 10.1016/j.pdpdt.2020.102038. Epub 2020 Oct 1.
Traditional chemomechanical treatment procedures are an indispensable part of endodontic treatment, however, additional treatment approaches such as antimicrobial photodynamic therapy (aPDT) may also be recommended for the elimination of residual microorganisms. In this study, the disinfection efficiency of aPDT performed using methylene blue (MB), toluidine blue (TB), and tetra 2-mercaptopyridine substituted zinc phthalocyanine (TM-ZnPc) was compared in the roots contaminated with Enterococcus faecalis (E. faecalis).
Forty-nine teeth with a single root and canal were included in this study. The roots were sterilized, and inoculated with E. faecalis. The roots were kept in an incubator for 30 days to form the biofilm. Forty-five teeth were prepared up to the F3 file of the ProTaperNext system under 2.5 % NaOCL irrigation. The samples were divided into three groups according to the type of used photosensitizer (PS) (n = 15); MB (313 μM), TB (327 μM), and TM-ZnPc (6μM). All PSs were irradiation with a light-emitting diode (LED) lamp (630 nm, 2-4 mW/cm²) for the 60 s. Two microbiological samples of the intracanal content were taken (one before and one immediately after additional aPDT in all groups) using sterile paper points. The colony-forming units per milliliter (CFU/mL) were calculated after 24 h of incubation at 37 °C.
After all aPDT protocols, intracanal bacterial load decreased significantly compared to the amount after chemomechanical preparation (P < 0.05). No significant difference in the reduction in intracanal bacterial load was found between the PSs (P < 0.05).
In the current study, the aPDT protocol performed with TM-ZnPc provided similar antimicrobial efficacy, although it was used at a lower concentration compared to MB and TB. Therefore, the use of TM-ZnPc in intra-canal disinfection in endodontics seems promising.
传统的化学机械处理程序是牙髓治疗不可或缺的一部分，然而，也可能推荐使用抗菌光动力疗法（aPDT）等附加治疗方法来消除残留的微生物。在这项研究中，比较了亚甲蓝（MB）、甲苯胺蓝（TB）和四（2-巯基吡啶）取代锌酞菁（TM-ZnPc）在受粪肠球菌（E. faecalis）污染的根管中的消毒效率。
本研究纳入了 49 颗具有单根和根管的牙齿。将这些根部分别进行灭菌，并接种粪肠球菌。将这些根在孵育箱中放置 30 天以形成生物膜。将 45 颗牙齿用 2.5%次氯酸钠冲洗下预备至 ProTaperNext 系统的 F3 锉。根据使用的光敏剂（PS）的类型将这些样本分为三组（n = 15）：MB（313 μM）、TB（327 μM）和 TM-ZnPc（6 μM）。所有 PS 均用发光二极管（LED）灯（630nm，2-4mW/cm²）照射 60s。所有组均在根管内直接进行一次额外的 aPDT，分别在处理前和处理后立即用无菌纸尖采集根管内内容物的两个微生物样本。将样本在 37°C 下孵育 24 小时后，计算每毫升的菌落形成单位（CFU/mL）。
所有 aPDT 方案后，根管内细菌负荷量与化学机械预备后相比均显著降低（P < 0.05）。PS 之间在根管内细菌负荷量减少方面无显著差异（P < 0.05）。
在本研究中，与 MB 和 TB 相比，尽管 TM-ZnPc 的使用浓度较低，但使用 TM-ZnPc 进行的 aPDT 方案提供了相似的抗菌效果。因此，在牙髓治疗中使用 TM-ZnPc 进行根管内消毒似乎很有前景。
