探索不同的光敏剂以优化抗菌光动力疗法中从感染根管中消除粪肠球菌浮游和生物膜形式。
Exploring different photosensitizers to optimize elimination of planktonic and biofilm forms of Enterococcus faecalis from infected root canal during antimicrobial photodynamic therapy.
机构信息
Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran; Department of Medical Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Photodiagnosis Photodyn Ther. 2018 Dec;24:206-211. doi: 10.1016/j.pdpdt.2018.09.014. Epub 2018 Sep 29.
BACKGROUND
Despite the high success rate of endodontic treatment, failure may occur in some cases. In this case, Enterococcus faecalis is the most common species in endodontic treatment failure and post-treatment apical periodontitis. Therefore, a new adjunctive strategy is needed for the prevention of endodontic infections due to E. faecalis. The aim of the present study was to compare the antimicrobial and anti-biofilm activities of different common photosensitizers (PSs) for use in antimicrobial photodynamic therapy (aPDT) against E. faecalis.
MATERIALS AND METHODS
E. faecalis strain ATCC 29212 was used as the tested strain and methylene blue (MB), toluidine blue O (TBO), indocyanine green (ICG), and curcumin (CUR) were used as PSs. Irradiation was carried out using diode laser and light emitting diode (LED) at wavelengths related to the above PSs. Then, antimicrobial and anti-biofilm activities were measured using the microbial viability assay and crystal violet test, respectively.
RESULTS
aPDT with using the above PSs significantly decreased the CFU/mL count of E. faecalis compared to the control group (P < 0.05). The killing percentage of E. faecalis via PS mediated aPDT was 99.6%, 98.2%, 85.1%, and 65.0% for CUR, ICG, TBO, and MB, respectively. aPDT using the above PSs significantly decreased the biofilm formation ability of E. faecalis compared to the control group (P < 0.05). The biofilm reduction percentage of the PSs was 68.4%, 62.9%, 59.0%, and 47.6% for CUR, ICG, TBO, and MB, respectively.
CONCLUSION
CUR and ICG mediated aPDT exhibited considerably more antimicrobial activity than other PSs, while TBO and MB demonstrated weaker anti-biofilm effects against E. faecalis compared to other PSs.
背景
尽管牙髓治疗的成功率很高，但在某些情况下仍可能失败。在这种情况下，粪肠球菌是牙髓治疗失败和治疗后根尖周炎最常见的物种。因此，需要一种新的辅助策略来预防粪肠球菌引起的牙髓感染。本研究的目的是比较不同常见光敏剂（PS）在抗粪肠球菌的抗菌光动力疗法（aPDT）中的抗菌和抗生物膜活性。
材料和方法
粪肠球菌菌株 ATCC 29212 用作测试菌株，亚甲蓝（MB）、甲苯胺蓝 O（TBO）、吲哚菁绿（ICG）和姜黄素（CUR）用作 PS。使用二极管激光和发光二极管（LED）在与上述 PS 相关的波长下进行照射。然后，使用微生物活力测定法和结晶紫试验分别测量抗菌和抗生物膜活性。
结果
与对照组相比，使用上述 PS 的 aPDT 显著降低了粪肠球菌的 CFU/mL 计数（P<0.05）。PS 介导的 aPDT 对粪肠球菌的杀灭率分别为 CUR、ICG、TBO 和 MB 的 99.6%、98.2%、85.1%和 65.0%。与对照组相比，使用上述 PS 的 aPDT 显著降低了粪肠球菌的生物膜形成能力（P<0.05）。PS 的生物膜减少率分别为 CUR、ICG、TBO 和 MB 的 68.4%、62.9%、59.0%和 47.6%。
结论
CUR 和 ICG 介导的 aPDT 表现出比其他 PS 更强的抗菌活性，而 TBO 和 MB 对粪肠球菌的抗生物膜作用弱于其他 PS。
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