Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China; College of Food Science and Engineering, Jilin Agricultural University, Changchun 130117, China.
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China.
J Pharm Biomed Anal. 2021 Jan 5;192:113648. doi: 10.1016/j.jpba.2020.113648. Epub 2020 Sep 23.
Direct analysis in real time ionization source coupled with quadrupole orbitrap mass spectrometry (DART-Q-Orbitrap MS) was applied to analyze the Schisandra chinensis (S. chinensis) and Schisandra sphenanthera (S. sphenanthera) samples. The experimental condition including the ionization gas and gas temperature were optimized to obtain the best performance. The DART-MS analysis was operated using helium at 250 °C. The partial least squares discriminant analysis (PLS-DA) was conducted based on the DART-MS data to explore the differences between S. chinensis and S. sphenanthera samples. The clear separation between groups was observed in the PLS-DA score plot, indicating the chemome diversity of these two samples. Then 8 compounds that contribute most to the sample classification were selected and annotated, and the intensity change tendency of these compounds was similar to that obtained by the high-performance liquid chromatography (HPLC) method. Besides, these two species can also be discriminated by examining the existence of the compound anwulignan at m/z 328.1656 in this study. Our results show that DART-MS is a powerful analytical tool with the merit of rapid analysis speed, easy to handle, low consumption of organic solvent, and has the great potential for rapid detection and discrimination of S. chinensis and S. sphenanthera. It is expected that the established method could provide a rapid, reliable method for the quality assessment of Schisandra species, and expand this method to the analysis of other herbal medicines.
直接分析实时电离源与四极轨道阱质谱联用（DART-Q-Orbitrap MS）被应用于分析五味子（S. chinensis）和华中五味子（S. sphenanthera）样品。优化了包括电离气体和气体温度在内的实验条件，以获得最佳性能。DART-MS 分析在 250°C 下使用氦气进行。基于 DART-MS 数据进行偏最小二乘判别分析（PLS-DA），以探索 S. chinensis 和 S. sphenanthera 样品之间的差异。在 PLS-DA 得分图中观察到组间的明显分离，表明这两个样品的化学多样性。然后选择并注释了对样品分类贡献最大的 8 种化合物，这些化合物的强度变化趋势与高效液相色谱（HPLC）方法获得的趋势相似。此外，通过检查本研究中 m/z 328.1656 处化合物 anwulignan 的存在，也可以区分这两个物种。研究结果表明，DART-MS 是一种强大的分析工具，具有快速分析速度、易于操作、有机溶剂消耗低的优点，具有快速检测和区分五味子和华中五味子的巨大潜力。预计建立的方法可以为五味子属的质量评估提供快速、可靠的方法，并将该方法扩展到其他草药的分析中。
