Demirel Cem, Turkoz Dursun, Yazicioglu Irem Melike, Cokluk Cengiz
Department of Neurosurgery, University of Health Sciences, Samsun Education and Research Hospital, Samsun, Turkey.
Department of Neurosurgery, University of Health Sciences, Samsun Education and Research Hospital, Samsun, Turkey.
World Neurosurg. 2021 Jan;145:e141-e148. doi: 10.1016/j.wneu.2020.09.140. Epub 2020 Oct 1.
The aim of the study was to evaluate the effect of systemically administrated curcumin on the prevention of peridural fibrotic tissue and adhesion formation in a rat laminectomy model.
Thirty-two Wistar albino rats were randomly selected and equally divided into 4 groups as follows: negative control group (group I) did not undergo operation; positive control group (group II) underwent laminectomy without treatment; group III (low-dose curcumin; 100 mg/kg); and group IV (high-dose curcumin; 200 mg/kg). Curcumin was administered intraperitoneally per day for 7 days after surgery starting from day 0. Twenty-eight days after surgery, T12 and L4 vertebral columns, paraspinal tissues, and epidural scar tissue were dissected en bloc and prepared for histopathologic examinations. All specimens were examined for inflammation, epidural fibrosis (EF), foreign body reaction, medulla spinalis retraction, granulation tissue, and arachnoid involvement. A Kruskal-Wallis test followed by a Dunn multiple comparison test were used for statistical analysis, and a P value <0.05 was considered as statistically significant.
Curcumin treatment significantly reduced inflammation, foreign body reaction, granulation tissue formation, medulla spinalis retraction, and EF formation compared with positive control group (P < 0.05); however, no significant differences were found between the 2 groups that received different doses of curcumin.
The results of the present study showed that systemic administration of curcumin was effective in reducing EF formation, inflammation, granulation tissue formation, medulla spinalis retraction, and foreign body reaction in the laminectomy area. Our results suggest that antiinflammatory activities of curcumin are beneficial for attenuation of EF formation.
本研究旨在评估全身给予姜黄素对大鼠椎板切除模型中硬膜外纤维化组织及粘连形成的预防作用。
随机选取32只Wistar白化大鼠，平均分为4组：阴性对照组（I组）不进行手术；阳性对照组（II组）行椎板切除术后不进行处理；III组（低剂量姜黄素组；100 mg/kg）；IV组（高剂量姜黄素组；200 mg/kg）。术后从第0天开始，每天腹腔注射姜黄素，共7天。术后28天，将T12和L4椎体、椎旁组织及硬膜外瘢痕组织整块切除，准备进行组织病理学检查。所有标本均检查炎症、硬膜外纤维化（EF）、异物反应、脊髓回缩、肉芽组织及蛛网膜受累情况。采用Kruskal-Wallis检验及Dunn多重比较检验进行统计学分析，P值<0.05认为具有统计学意义。
与阳性对照组相比（P < 0.05），姜黄素治疗显著减轻了炎症、异物反应、肉芽组织形成、脊髓回缩及EF形成；然而，接受不同剂量姜黄素的两组之间未发现显著差异。
本研究结果表明，全身给予姜黄素可有效减少椎板切除区域EF形成、炎症、肉芽组织形成、脊髓回缩及异物反应。我们的结果提示，姜黄素的抗炎活性有利于减轻EF形成。
