Materials Sciences and Technology Division, CSIR-North East Institute of Science and Technology, Jorhat 785006, Assam, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad-201002, India.
Department of Biomedical Engineering, Indian Institute of Technology Hyderabad, India.
J Photochem Photobiol B. 2020 Nov;212:112028. doi: 10.1016/j.jphotobiol.2020.112028. Epub 2020 Sep 18.
Graphene, which is a unique 2D nanomaterials has received widespread attention for photothermal therapy (PTT) application. Here, we have designed the nanocomposite using polydopamine (PDA) functionalized reduced graphene oxide (rGO) nanosheets and bimetallic AuPd nanoparticles (NPs). The bimetallic AuPd nanoparticles decorated PDA functionalized rGO (AuPd-rGO/PDA) nanocomposite is synthesized by simple chemical reduction technique resulting in an average size of AuPd bimetallic nanostructure of 4.18 nm. The photothermal activity of the AuPd-rGO/PDA nanocomposite is explored under the irradiation of near infrared (NIR) laser sources of wavelength 915 nm. The temperature rises nearly 51 ± 3 °C within 3 min of irradiation NIR laser light resulting in the ablation of MDAMB-231 cancer cells up to concentration of 25 μg mL of AuPd-rGO/PDA nanocomposite. This high performance of the ablation of cancer cells by photothermal therapy technique was facilitated using a low concentration of the nanocomposite by the synergistic effects of the bimetallic AuPd as well as rGO and PDA functionalization. The AuPd-rGO/PDA nanocomposite demonstrated the high biocompatibility towards normal healthy cell lines (L929) and exhibits survival efficiency of more than 85%. We also demonstrated the biocompatibility of the AuPd-rGO/PDA nanocomposite materials on the zebrafish embryos (Danio rerio). This work thus illustrates that the AuPd-rGO/PDA nanocomposite could behave as favourable nanoplatform for tumor therapeutics.
石墨烯是一种独特的二维纳米材料，因其在光热治疗（PTT）中的应用而受到广泛关注。在这里，我们设计了一种使用聚多巴胺（PDA）功能化还原氧化石墨烯（rGO）纳米片和双金属 AuPd 纳米粒子（NPs）的纳米复合材料。通过简单的化学还原技术合成了双金属 AuPd 纳米粒子修饰的 PDA 功能化 rGO（AuPd-rGO/PDA）纳米复合材料，得到的 AuPd 双金属纳米结构的平均尺寸为 4.18nm。在近红外（NIR）激光波长 915nm 的照射下，研究了 AuPd-rGO/PDA 纳米复合材料的光热活性。在近红外激光照射 3 分钟内，温度升高近 51±3°C，导致浓度高达 25μg mL 的 AuPd-rGO/PDA 纳米复合材料的 MDAMB-231 癌细胞消融。这种通过光热疗法技术高效消融癌细胞的性能是通过协同作用的双金属 AuPd 以及 rGO 和 PDA 功能化，使用低浓度的纳米复合材料来实现的。AuPd-rGO/PDA 纳米复合材料对正常健康细胞系（L929）表现出高生物相容性，存活率超过 85%。我们还在斑马鱼胚胎（Danio rerio）上证明了 AuPd-rGO/PDA 纳米复合材料的生物相容性。这项工作表明，AuPd-rGO/PDA 纳米复合材料可以作为肿瘤治疗的有利纳米平台。
