Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Marine Food, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.
Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Marine Food, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.
Food Chem. 2021 Mar 15;340:128139. doi: 10.1016/j.foodchem.2020.128139. Epub 2020 Sep 25.
Chemical pretreatment of collagen raw materials is time-consuming and environmentally hazardous. Collagen extraction after fermentation pretreatment has not been reported. We extracted and characterized acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from Nile tilapia (Oreochromis niloticus) skin following fermentation and chemical treatments and comparatively evaluated the feasibility of fermentation. Fermentation-ASC (FASC) and fermentation-PSC (FPSC) yields (4.76 and 8.14 wt%, respectively) were slightly but not significantly higher than chemical-ASC (CASC) and chemical-PSC (CPSC) yields (4.27 and 7.60 wt%, respectively). All extracts were identified as type I collagens by SDS-PAGE and retained their triple helical structure well, as confirmed through Fourier transform infrared spectroscopy. All collagen microstructures under scanning electron microscopy were multi-layered aggregates. These collagens also had similar biochemical properties (i.e. denatured between 36.5 and 37.1 °C, high soluble at pH 1-4 and at <3% [w/v] NaCl). Therefore, fermentation method is a viable alternative for pretreating collagen extraction materials.
胶原原料的化学预处理既耗时又对环境有害。发酵预处理后胶原的提取尚未见报道。我们从尼罗罗非鱼（Oreochromis niloticus）皮中提取并表征了酸溶性胶原（ASC）和胃蛋白酶溶性胶原（PSC），分别进行了发酵和化学处理，并比较了发酵的可行性。发酵-ASC（FASC）和发酵-PSC（FPSC）的产率（分别为 4.76%和 8.14%）略高于化学-ASC（CASC）和化学-PSC（CPSC）的产率（分别为 4.27%和 7.60%），但差异不显著。所有提取物均通过 SDS-PAGE 鉴定为 I 型胶原蛋白，并通过傅里叶变换红外光谱证实其保持了良好的三螺旋结构。所有胶原的微观结构在扫描电子显微镜下均为多层聚集物。这些胶原蛋白还具有相似的生化特性（即在 36.5 至 37.1°C 变性，在 pH1-4 和 <3%[w/v]NaCl 下高度溶解）。因此，发酵法是预处理胶原提取材料的可行替代方法。
