Zhang Qi, Hou Shufang, Liu Yanmei, Du Jia, Jia Yongkang, Yang Qiushi, Xu Tingting, Takagi Yasuaki, Li Dapeng, Zhang Xi
College of Fisheries, National Demonstration Center for Experimental Aquaculture Education, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Hubei Provincial Engineering Laboratory for Pond Aquaculture, Huazhong Agricultural University, Wuhan 430070, China.
Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041-8611, Hokkaido, Japan.
Foods. 2024 Sep 13;13(18):2901. doi: 10.3390/foods13182901.
The southern catfish () is an economically important carnivorous freshwater fish in China. In this study, we compared the properties of skin collagen from southern catfish fed with raw food (RF) and cooked food (CF). The skin collagen yield in the RF group (8.66 ± 0.11%) was significantly higher than that of the CF group (8.00 ± 0.27%). SDS-PAGE, circular dichroism spectroscopy, and FTIR analyses revealed that the collagen extracted from southern catfish skin in both groups was type I collagen, with a unique triple helix structure and high purity. The thermal denaturation temperature of collagen in the RF group (35.20 ± 0.11 °C) was significantly higher than that of the CF group (34.51 ± 0.25 °C). The DPPH free radical scavenging rates were 68.30 ± 2.41% in the RF collagen and 61.78 ± 3.91% in the CF collagen, which was higher than that found in most fish collagen. Both the RF and CF groups had high ability to form fibrils in vitro. Under the same conditions, the CF group exhibited faster fibril formation and a thicker fibril diameter ( < 0.05). In addition, the RF group exhibited significantly higher expression of compared to the CF group. These results indicated that feeding southern catfish raw food contributed to collagen production, and the collagen from these fish may have potential in biomaterial applications.
南方鲇（）是中国一种具有重要经济价值的肉食性淡水鱼。在本研究中，我们比较了喂食生食（RF）和熟食（CF）的南方鲇皮肤胶原蛋白的特性。RF组的皮肤胶原蛋白产量（8.66±0.11%）显著高于CF组（8.00±0.27%）。SDS-PAGE、圆二色光谱和FTIR分析表明，两组从南方鲇皮肤中提取的胶原蛋白均为I型胶原蛋白，具有独特的三螺旋结构且纯度高。RF组胶原蛋白的热变性温度（35.20±0.11℃）显著高于CF组（34.51±0.25℃）。RF胶原蛋白的DPPH自由基清除率为68.30±2.41%，CF胶原蛋白的为61.78±3.91%，高于大多数鱼类胶原蛋白。RF组和CF组在体外都有较高的形成原纤维的能力。在相同条件下，CF组原纤维形成更快且原纤维直径更粗（<0.05）。此外，RF组的表达明显高于CF组。这些结果表明，喂食南方鲇生食有助于胶原蛋白的产生，这些鱼的胶原蛋白在生物材料应用中可能具有潜力。
