State Key Laboratory of Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
State Key Laboratory of Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China; The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, 450052, Henan, China.
Virology. 2020 Dec;551:16-25. doi: 10.1016/j.virol.2020.08.012. Epub 2020 Sep 28.
Knowledge about the special characteristics of HIV-1 envelope (env) glycoproteins in rare individuals developing >90% neutralization breadth in Chinese subtype B' slow progressors may provide insights for vaccine design against local viruses. We performed a cross-sectional analysis on 7 samples. We tested the neutralization breadth and geometric mean ID50 titers (GMTs) of these samples, and divided them into hBCN+ and hBCN- group according to whether their neutralization breadth >90%. We obtained env sequences in these samples through single genome amplification (SGA) assay. By comparing with hBCN-, subtype B chronically infected group (B-SP), and Chinese subtype B group (B-Database), we analyzed the characteristics of the env sequences of hBCN+ group. Longer V1 and V4 regions with more glycosylation sites were found in hBCN+ samples compared to hBCN- samples. Further analysis compared to B-SP and B-Database showed that hBCN+ group exhibited unique extra-long V1 region containing higher proportion of N-glycan sites and additional cysteines.
对中国 B' 亚型慢进展者中少数能产生 >90%中和广度的个体中 HIV-1 包膜 (env) 糖蛋白的特殊特征的了解，可能为针对当地病毒的疫苗设计提供思路。我们对 7 个样本进行了横断面分析。我们测试了这些样本的中和广度和几何平均 ID50 滴度（GMT），并根据中和广度是否 >90%将它们分为 hBCN+和 hBCN-组。我们通过单基因组扩增 (SGA) 测定从这些样本中获得了 env 序列。通过与 hBCN-、慢性感染 B 亚型组（B-SP）和中国 B 亚型组（B-Database）进行比较，我们分析了 hBCN+组 env 序列的特征。与 hBCN-样本相比，hBCN+样本的 V1 和 V4 区更长，糖基化位点更多。与 B-SP 和 B-Database 的进一步分析表明，hBCN+组表现出独特的超长 V1 区，含有更高比例的 N-糖基化位点和额外的半胱氨酸。
