从两名东印度族散发性帕金森病患者中诱导生成多能干细胞（NIMHi002 - A和NIMHi003 - A）。
Generation of induced pluripotent stem cells (NIMHi002-A and NIMHi003-A) from two sporadic Parkinson's disease patient of East Indian ethnicity.
作者信息
Datta Indrani, Jagtap Soham, Yadav Ravi, Pal Pramod Kumar
机构信息
Department of Biophysics, National Institute of Mental Health and Neurosciences, Bengaluru 560029, India.
Department of Biophysics, National Institute of Mental Health and Neurosciences, Bengaluru 560029, India.
出版信息
Stem Cell Res. 2020 Dec;49:101995. doi: 10.1016/j.scr.2020.101995. Epub 2020 Sep 17.
Epidemiological studies suggest that about 95% of PD have a sporadic component. We have generated induced pluripotent stem cells (iPSCs) using Sendai-virus reprogramming-method from peripheral blood mononuclear cells of two sporadic PD-patient of East-Indian ethnicity carrying no PD-related gene mutations. PD diagnosis was performed using Unified Parkinson's Disease rating scale (UPDRS) score and confirmed by [F]fluoro-L-dopa [F-DOPA] positron emission tomography (F-DOPA PET). The iPSC lines were characterized for self-renewal and pluripotency. These generated lines will provide a valuable resource to understand the pathophysiology of this disease and a drug-screening platform.
流行病学研究表明，约95%的帕金森病具有散发性成分。我们使用仙台病毒重编程方法，从两名没有帕金森病相关基因突变的东印度族裔散发性帕金森病患者的外周血单个核细胞中生成了诱导多能干细胞（iPSC）。使用统一帕金森病评定量表（UPDRS）评分进行帕金森病诊断，并通过[F]氟代左旋多巴[F-DOPA]正电子发射断层扫描（F-DOPA PET）进行确认。对iPSC系进行了自我更新和多能性特征分析。这些生成的细胞系将为理解该疾病的病理生理学提供宝贵资源，并为药物筛选提供平台。
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