Fishery College, Guangdong Ocean University, Zhanjiang 524025, China.
Fishery College, Guangdong Ocean University, Zhanjiang 524025, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang 524088, China; Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Zhanjiang 524088, China.
Comp Biochem Physiol B Biochem Mol Biol. 2021 Feb-Mar;252:110512. doi: 10.1016/j.cbpb.2020.110512. Epub 2020 Oct 2.
The cholinergic anti-inflammatory pathway has been identified as a reflex monitoring system that contributes to the physiological and pathological regulation of cytokines. Nicotinic acetylcholine receptor (nAChR) plays an important role in immune regulation as a key molecule in neuronal communication. In this work, we investigated the characteristics and functions of a novel nAChR β gene identified from the pearl oyster Pinctada fucata martensii (PmnAChR-β). PmnAChR-β displays structural similarities to nAChR molecules described in mammals, including a typical neurotransmitter-gated ion-channel ligand binding domain (LBD) and transmembrane (TM) domain. The result of phylogenetic analysis speculated that nAChR-β in Mollusca, Chordata and Arthropoda were separated into three branches. The LBD of PmnAChR-β was highly conserved, but its TM was variable. PmnAChR-β was highly expressed in eggs and fertilized eggs and had the most abundant mRNA expression in the gills of pearl oyster. The expression of PmnAChR-β mRNA was dramatically upregulated 12 h after lipopolysaccharide stimulation. Furthermore, PmnAChR-β was highly expressed at 12 h and 6-18 d after transplantation in hemocytes. Pm-miR-516b-5p was identified as the regulatory microRNA of PmnAChR-β. These results indicated that PmnAChR-β may be an important component of the cholinergic anti-inflammatory pathway and participates in the immune regulation process of pearl oysters.
胆碱能抗炎通路已被确定为一种反射监测系统，有助于细胞因子的生理和病理调节。烟碱型乙酰胆碱受体（nAChR）作为神经元通讯的关键分子，在免疫调节中发挥重要作用。在这项工作中，我们研究了从珍珠贝 Pinctada fucata martensii（PmnAChR-β）中鉴定出的新型 nAChR β 基因的特征和功能。PmnAChR-β与哺乳动物中描述的 nAChR 分子具有结构相似性，包括典型的神经递质门控离子通道配体结合域（LBD）和跨膜（TM）域。系统发育分析的结果推测，软体动物、脊索动物和节肢动物中的 nAChR-β 分为三个分支。PmnAChR-β的 LBD 高度保守，但 TM 是可变的。PmnAChR-β在卵子和受精卵中高度表达，在珍珠贝的鳃中 mRNA 表达最丰富。脂多糖刺激后 12 h，PmnAChR-β 的 mRNA 表达显著上调。此外，PmnAChR-β在血细胞中的表达在移植后 12 h 和 6-18 d 时高度表达。Pm-miR-516b-5p 被鉴定为 PmnAChR-β 的调节 microRNA。这些结果表明，PmnAChR-β可能是胆碱能抗炎通路的重要组成部分，参与珍珠贝的免疫调节过程。
