Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan; LO-Sheng Hospital, Ministry of Health and Welfare, Taipei, Taiwan; School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan.
Int J Biol Macromol. 2020 Dec 15;165(Pt A):1604-1613. doi: 10.1016/j.ijbiomac.2020.09.227. Epub 2020 Oct 1.
WSG is a water soluble polysaccharides isolated from Ganoderma lucidum. In this study, we showed that WSG, a glucose-rich polysaccharide with an average molecular mass of approximately 1000 kDa, effectively inhibited cell viability and mobility of lung cancer cells. Functional studies revealed that WSG reduced phosphorylation of ERK1/2 in cells upon either EGF or TGFβ stimulation. WSG also inhibited phosphorylation of multiple intracellular signaling molecules such as FAK, AKT and Smad2. Mechanistically, we demonstrated that WSG induced degradation of TGFβ and EGF receptors via proteasome and lysosome, respectively. Moreover, we found that WSG significantly suppressed lung tumor growth, reduced the size of metastatic nodules in the lungs and prolonged the survival of LLC1-bearing mice. Our findings suggested that WSG may have potential as a therapeutic intervention for treatment of lung cancer.
WSG 是一种从灵芝中分离得到的水溶性多糖。在这项研究中，我们表明，WSG 是一种葡萄糖丰富的多糖，平均分子量约为 1000 kDa，可有效抑制肺癌细胞的活力和迁移。功能研究表明，WSG 可降低 EGF 或 TGFβ刺激时细胞中 ERK1/2 的磷酸化。WSG 还抑制了 FAK、AKT 和 Smad2 等多种细胞内信号分子的磷酸化。从机制上讲，我们证明 WSG 通过蛋白酶体和溶酶体分别诱导 TGFβ 和 EGF 受体的降解。此外，我们发现 WSG 可显著抑制肺肿瘤生长，减少肺转移结节的大小，并延长 LLC1 荷瘤小鼠的生存时间。我们的研究结果表明，WSG 可能具有作为治疗肺癌的治疗干预的潜力。
