Department of Animal Genetics and Breeding, Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS), Hisar, India.
Dairy Cattle Breeding Division, ICAR- National Dairy Research Institute, 132001, Haryana, India.
Res Vet Sci. 2020 Dec;133:194-201. doi: 10.1016/j.rvsc.2020.09.013. Epub 2020 Sep 21.
The present study was conducted on Karan Fries cows to identify SNPs in breast cancer 1 (BRCA1) gene and their association with inflammation of mammary gland and production traits. Chi square analysis showed significant association of both SNPs i.e. G43737229T (P < 0.01) and G43761121A (P < 0.05) with incidence of clinical mastitis. Logistic modeling showed that GG genotype cows of G43737229T SNP was less susceptible to mastitis in comparison to TT genotype cows as the odds ratio of TT against GG for the non-incidence of mastitis was less i.e. 0.23. Whereas, cows with genotype AA of G43761121A SNP were less prone to clinical mastitis in comparison to GG genotype cows. G-T haplotype and GGTT combined genotype exhibited maximum mastitis incidences. Least squares analysis revealed significant association of G43737229T SNP with total milk yield (P < 0.01), 305 days milk yield (P < 0.01) and lactation length (P < 0.05). The TT and GT genotyped Karan Fries cows were associated with higher milk yields during first and second lactation, as well as with first lactation length. These observations on SNPs, genotypes and combined genotypes of BRCA1 gene and their differential association with the incidence of mastitis and production traits can be utilized as an aid to selection for simultaneous improvement of both antagonistic traits.
本研究以卡兰弗里奶牛为研究对象，旨在鉴定乳腺癌 1 号（BRCA1）基因中的单核苷酸多态性及其与乳腺炎症和生产性状的关系。卡方分析显示，两个 SNP（即 G43737229T（P<0.01）和 G43761121A（P<0.05））与临床乳腺炎的发生率均存在显著关联。逻辑回归模型显示，与 TT 基因型奶牛相比，G43737229T SNP 的 GG 基因型奶牛发生乳腺炎的风险较低，因为 TT 基因型奶牛发生乳腺炎的可能性（OR）为 GG 基因型奶牛的 0.23。相比之下，G43761121A SNP 的 AA 基因型奶牛发生临床乳腺炎的风险低于 GG 基因型奶牛。G-T 单倍型和 GGTT 组合基因型表现出最高的乳腺炎发病率。最小二乘分析显示，G43737229T SNP 与总奶产量（P<0.01）、305 天奶产量（P<0.01）和泌乳期长度（P<0.05）显著相关。TT 和 GT 基因型的卡兰弗里奶牛与第一和第二泌乳期的更高产奶量以及第一泌乳期的更长时间有关。这些关于 BRCA1 基因的 SNP、基因型和组合基因型及其与乳腺炎发生率和生产性状的差异关联的观察结果可用于辅助选择，以同时改善这两种拮抗性状。
