Liu Yanger, Guo Mingzhang, Du Ruoxi, Chi Jiani, He Xiaoyun, Xie Zixin, Huang Kunlun, Luo Yunbo, Xu Wentao
Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing, 100083, China; Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety) (MOA), College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing, 100083, China.
Biosens Bioelectron. 2020 Dec 15;170:112660. doi: 10.1016/j.bios.2020.112660. Epub 2020 Sep 28.
As optical reporting elements, fluorescent proteins are extensively used in whole-cell microbial biosensors. However, the use of these optical reporters is limited in opaque media such as soil. This study described a method utilizing gas as a reporting signal that could be used for the rapid on-site detection of mercury in soil. In this biosensor, the MerR protein could capture mercury ions and then bind the promoter of the efe gene to initiate the synthesis of the ethylene (CH)-forming enzyme that produced the gas. The research showed that the mercury ion concentrations could be converted into CH gas signals, which were quantified using a handheld CH sensor. By optimizing the biosensor to improve its anti-interference ability in the system, it could detect mercury ion concentrations in the soil ranging from 0.2 to 20 mg/kg within 45 min, effectively reflecting whether the mercury pollution in the soil exceeded the limit standard. This study provides a simple, inexpensive, and portable method for the on-site detection of soil pollutants.
作为光学报告元件，荧光蛋白在全细胞微生物生物传感器中被广泛应用。然而，这些光学报告器在诸如土壤等不透明介质中的使用受到限制。本研究描述了一种利用气体作为报告信号的方法，该方法可用于土壤中汞的快速现场检测。在这种生物传感器中，MerR蛋白可以捕获汞离子，然后结合efe基因的启动子以启动产生气体的乙烯（CH）形成酶的合成。研究表明，汞离子浓度可以转化为CH气体信号，使用手持式CH传感器对其进行定量。通过优化生物传感器以提高其在系统中的抗干扰能力，它可以在45分钟内检测土壤中0.2至20mg/kg的汞离子浓度，有效反映土壤中的汞污染是否超过限量标准。本研究为土壤污染物的现场检测提供了一种简单、廉价且便携的方法。
