College of Sciences, China Agricultural University, Beijing, China.
Risk Assessment Laboratory for Bee Products Quality and Safety of Ministry of Agriculture, Institute of Agricultural Research, Chinese Academy of Agricultural Sciences, Beijing, China.
J Hazard Mater. 2021 Feb 15;404(Pt A):124083. doi: 10.1016/j.jhazmat.2020.124083. Epub 2020 Sep 23.
Boscalid is a persistent fungicide that is frequently detected in surface waters and may be neurotoxic to aquatic organisms. Herein, we evaluated the effects of environmentally relevant boscalid concentrations to zebrafish to explore its potentially neurotoxic mechanisms of effect. Behavioral responses (swimming, phototaxis, and predation), histopathology, transcriptomics, biochemical parameter analysis and gene expression of larval and adult zebrafish following boscalid treatment were assessed. We found that boscalid significantly inhibited the locomotor ability and phototactic response of larvae after an 8-d exposure, and altered the locomotor activity, predation trajectories and ability in adults after a 21-d exposure. It was noted that predation rates of zebrafish were significantly decreased by 30% and 100% after exposure to 0.1 and 1.0 mg/L boscalid, respectively. Adverse alterations in the cell differentiation of eyes and brain injury were also observed in both larvae and adults following boscalid exposure. The expression of genes related to neurodevelopment, neurotransmission, eye development, and visual function, in conjunction with RNA-Seq results, indicated that boscalid may impair visual phototransduction and nervous system processes in larval zebrafish. Conclusively, boscalid exposure may affect the neurobehavioral response of zebrafish by impairing proper visual and nervous system function.
波尔多液是一种持久性杀菌剂，经常在地表水中被检测到，可能对水生生物具有神经毒性。在此，我们评估了环境相关浓度的波尔多液对斑马鱼的影响，以探索其潜在的神经毒性作用机制。评估了斑马鱼在波尔多液处理后的行为反应（游泳、趋光性和捕食）、组织病理学、转录组学、生化参数分析以及幼鱼和成鱼的基因表达。我们发现，波尔多液在 8 天暴露后显著抑制了幼鱼的运动能力和趋光反应，在 21 天暴露后改变了成鱼的运动活性、捕食轨迹和能力。值得注意的是，暴露于 0.1 和 1.0mg/L 波尔多液后，斑马鱼的捕食率分别显著降低了 30%和 100%。在幼鱼和成鱼中，还观察到眼睛细胞分化和脑损伤的不良改变。与神经发育、神经传递、眼睛发育和视觉功能相关的基因的表达，以及 RNA-Seq 结果表明，波尔多液可能损害幼鱼的视觉光转导和神经系统过程。总之，波尔多液暴露可能通过损害适当的视觉和神经系统功能来影响斑马鱼的神经行为反应。
