Nakajima Takayuki, Otsuka Kenji, Tomizawa Sanae, Masubuchi Tamako, Yamaki Yumiko, Kamijo Kyoko, Yoshikawa Souichi, Takada Tomomi, Kokaji Yoshie, Watanabe Shui, Ohsawa Yoshihiro, Hashimoto Tsuneo
Tokyo Metropolitan Institute of Public Health.
Shokuhin Eiseigaku Zasshi. 2020;61(4):154-160. doi: 10.3358/shokueishi.61.154.
In the field of food analysis and regulation, different instruments are used to determine the accuracy of quantification values. This is essential, as inconsistencies in values are commonly encountered. To visualize the degree of these discrepancies in each food matrix, we compiled a validation study based on a routine method developed in our laboratory, for 121 pesticides in six agricultural products, namely the grapefruit, potato, paprika, cabbage, spinach, and brown rice. These were analyzed by GC-MS/MS and LC-MS/MS, and the results were compared mainly on the basis of trueness. According to the results of the validation study when using GC-MS/MS, of the 121 pesticides tested in each product class, the number of analytes that satisfied the criteria of the Japanese validation guidelines was 97 in grapefruit, 111 in potato, 110 in paprika, 118 in cabbage, 111 in spinach, and 63 in brown rice. In contrast, in the analysis of the same samples by using LC-MS/MS, the number of analytes that satisfied the criteria of the validation guidelines was 50 in grapefruit, 114 in potato, 103 in paprika, 112 in cabbage, 100 in spinach, and 103 in brown rice. Inconsistences in the differences of trueness were mainly attributed to matrix effects of each instrument, as well as to food matrices, of which the most diverged matrix was that of brown rice (over 20%).
在食品分析与监管领域，使用不同仪器来确定定量值的准确性。这至关重要，因为数值不一致的情况很常见。为了直观呈现每种食品基质中这些差异的程度，我们基于本实验室开发的常规方法，针对六种农产品（即葡萄柚、土豆、辣椒、卷心菜、菠菜和糙米）中的121种农药开展了一项验证研究。这些农产品通过气相色谱 - 串联质谱法（GC - MS/MS）和液相色谱 - 串联质谱法（LC - MS/MS）进行分析，结果主要基于准确性进行比较。根据使用GC - MS/MS进行验证研究的结果，在每个产品类别中测试的121种农药里，符合日本验证指南标准的分析物数量在葡萄柚中为97种，土豆中为111种，辣椒中为110种，卷心菜中为118种，菠菜中为111种，糙米中为63种。相比之下，在使用LC - MS/MS对相同样品进行分析时，符合验证指南标准的分析物数量在葡萄柚中为50种，土豆中为114种，辣椒中为103种，卷心菜中为112种，菠菜中为100种，糙米中为103种。准确性差异中的不一致主要归因于每种仪器的基质效应以及食品基质，其中差异最大的基质是糙米（超过20%）。
