Lattuada Enrico, Leo Manuela, Caprara Debora, Salvatori Luisa, Stoppacciaro Antonella, Sciortino Francesco, Filetici Patrizia
Department of Physics, Sapienza University of Rome, Rome, Italy.
Institute of Molecular Biology and Pathology - CNR, Sapienza University of Rome, Rome, Italy.
Front Pharmacol. 2020 Sep 4;11:01345. doi: 10.3389/fphar.2020.01345. eCollection 2020.
Novel DNA materials promise unpredictable perspectives for applications in cell biology. The realization of DNA-hydrogels built by a controlled association of DNA nanostars, whose binding can be tuned with minor changes in the nucleotide sequences, has been recently described. DNA hydrogels, with specific gelation properties that can be reassambled in desired culture media supplemented with drugs, RNA, DNA molecules and other bioactive compounds offer the opportunity to develop a novel nanomaterial for the delivery of single or multiple drugs in tumor tissues as an innovative and promising strategy. We provide here a comprehensive description of different, recently realized DNA-gels with the perspective of stimulating their biomedical application. Finally, we discuss the possibility to design sophisticated 3D tissue-like DNA-gels incorporating cell spheroids or single cells for the assembly of a novel kind of cellular matrix as a preclinical investigation for the implementation of tools for delivery of bioactive molecules.
新型DNA材料有望为细胞生物学应用带来不可预测的前景。最近已报道了通过DNA纳米星的可控缔合构建DNA水凝胶，其结合可通过核苷酸序列的微小变化进行调节。DNA水凝胶具有特定的凝胶化特性，可在添加药物、RNA、DNA分子和其他生物活性化合物的所需培养基中重新组装，为开发一种用于在肿瘤组织中递送单一或多种药物的新型纳米材料提供了机会，这是一种创新且有前景的策略。我们在此全面描述了不同的、最近实现的DNA凝胶，以期促进其生物医学应用。最后，我们讨论了设计包含细胞球体或单细胞的复杂三维组织样DNA凝胶以组装新型细胞基质的可能性，作为实施生物活性分子递送工具的临床前研究。
