Tu Yaoyao, Zheng Guangyong, Ding Guolian, Wu Yanting, Xi Ji, Ge Yingzhou, Gu Hangchao, Wang Yingyu, Sheng Jianzhong, Liu Xinmei, Jin Li, Huang Hefeng
Department of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, Shanghai, China.
Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China.
Front Physiol. 2020 Sep 8;11:1108. doi: 10.3389/fphys.2020.01108. eCollection 2020.
Women with polycystic ovarian syndrome (PCOS) often have a history of infertility and poor pregnancy outcome. The character of the lower genital tract (LGT) microbiome of these patients is still unknown. We collected both vaginal and cervical canal swabs from 47 PCOS patients (diagnosed by the Rotterdam Criteria) and 50 healthy reproductive-aged controls in this study. Variable regions 3-4 (V3-4) were sequenced and analyzed. Operational taxonomic unit (OTU) abundance was noted for all samples. Taxa that discriminated between PCOS and healthy women was calculated by linear discriminant analysis effect size (LEFSe). Results from 97 paired vaginal and cervical canal samples collected from 97 women [mean age 30 (±4 years)] were available for analysis. Using the Rotterdam Criteria, 47 women were diagnosed with PCOS (PCOS, = 47; control, = 50). There was no significant difference between cervical canal microbiome and vaginal microbiome from the same individual, however, spp. was less abundant in both vaginal and cervical canal microbiome of PCOS patients. Several non- taxa including , , and , were more abundant in the LGT microbiota of PCOS patients. There is a difference between the microorganism in the LGT of patients with PCOS and healthy reproductive-aged women.
患有多囊卵巢综合征（PCOS）的女性通常有不孕史且妊娠结局不佳。这些患者下生殖道（LGT）微生物群的特征仍不清楚。在本研究中，我们收集了47例PCOS患者（根据鹿特丹标准诊断）和50例健康育龄对照者的阴道和宫颈管拭子。对可变区3 - 4（V3 - 4）进行测序和分析。记录所有样本的操作分类单元（OTU）丰度。通过线性判别分析效应大小（LEFSe）计算区分PCOS患者和健康女性的分类群。从97名女性[平均年龄30（±4岁）]收集的97对阴道和宫颈管样本的结果可供分析。根据鹿特丹标准，47名女性被诊断为PCOS（PCOS组，n = 47；对照组，n = 50）。同一个体的宫颈管微生物群和阴道微生物群之间没有显著差异，然而，在PCOS患者的阴道和宫颈管微生物群中， spp. 的丰度均较低。包括 、 和 在内的几种非 分类群在PCOS患者的LGT微生物群中更为丰富。PCOS患者与健康育龄女性的LGT中的微生物存在差异。
