Garretto Andrea, Miller-Ensminger Taylor, Ene Adriana, Merchant Zubia, Shah Aashaka, Gerodias Athina, Biancofiori Anthony, Canchola Stacey, Canchola Stephanie, Castillo Emanuel, Chowdhury Tasnim, Gandhi Nikita, Hamilton Sarah, Hatton Kyla, Hyder Syed, Krull Koty, Lagios Demetrios, Lam Thinh, Mitchell Kennedy, Mortensen Christine, Murphy Amber, Richburg Joseph, Rokas Meghan, Ryclik Suzanne, Sulit Pauline, Szwajnos Thomas, Widuch Manuel, Willis Jessica, Woloszyn Mary, Brassil Bridget, Johnson Genevieve, Mormando Rita, Maskeri Laura, Batrich Mary, Stark Nicole, Shapiro Jason W, Montelongo Hernandez Cesar, Banerjee Swarnali, Wolfe Alan J, Putonti Catherine
Bioinformatics Program, Loyola University Chicago, Chicago, IL, United States.
Department of Biology, Loyola University Chicago, Chicago, IL, United States.
Front Microbiol. 2020 Sep 4;11:2094. doi: 10.3389/fmicb.2020.02094. eCollection 2020.
Urinary tract infections (UTIs) are one of the most common human bacterial infections. While UTIs are commonly associated with colonization by , members of this species also have been found within the bladder of individuals with no lower urinary tract symptoms (no LUTS), also known as asymptomatic bacteriuria. Prior studies have found that both uropathogenic (UPEC) strains and isolates that are not associated with UTIs encode for virulence factors. Thus, the reason(s) why sometimes causes UTI-like symptoms remain(s) elusive. In this study, the genomes of 66 isolates from adult female bladders were sequenced. These isolates were collected from four cohorts, including women: (1) without lower urinary tract symptoms, (2) overactive bladder symptoms, (3) urgency urinary incontinence, and (4) a clinical diagnosis of UTI. Comparative genomic analyses were conducted, including core and accessory genome analyses, virulence and motility gene analyses, and antibiotic resistance prediction and testing. We found that the genomic content of these 66 isolates does not correspond with the participant's symptom status. We thus looked beyond coli genomes to the composition of the entire urobiome and found that the presence of alone was not sufficient to distinguish between the urobiomes of individuals with UTI and those with no LUTS. Because presence, abundance, and genomic content appear to be weak predictors of UTI status, we hypothesize that UTI symptoms associated with detection of are more likely the result of urobiome composition.
尿路感染（UTIs）是人类最常见的细菌感染之一。虽然尿路感染通常与[某种细菌]的定植有关，但在没有下尿路症状（无LUTS）的个体膀胱中也发现了该菌种的成员，即无症状菌尿。先前的研究发现，尿路致病性[某种细菌]（UPEC）菌株和与尿路感染无关的[某种细菌]分离株都编码毒力因子。因此，[某种细菌]有时会引起类似尿路感染症状的原因仍然不明。在本研究中，对来自成年女性膀胱的66株[某种细菌]分离株的基因组进行了测序。这些分离株来自四个队列，包括女性：（1）没有下尿路症状，（2）膀胱过度活动症症状，（3）急迫性尿失禁，以及（4）临床诊断为尿路感染。进行了比较基因组分析，包括核心和辅助基因组分析、毒力和运动基因分析以及抗生素耐药性预测和测试。我们发现这66株[某种细菌]分离株的基因组内容与参与者的症状状态不相符。因此，我们将目光从大肠杆菌基因组扩展到整个泌尿微生物组的组成，发现仅[某种细菌]的存在不足以区分患有尿路感染的个体和没有下尿路症状的个体的泌尿微生物组。由于[某种细菌]的存在、丰度和基因组内容似乎是尿路感染状态的弱预测指标，我们推测与检测到[某种细菌]相关的尿路感染症状更可能是泌尿微生物组组成的结果。
