Li Miaomaio, Gašparovič Henrich, Weng Xing, Chen Si, Korduláková Jana, Jessen-Trefzer Claudia
Department of Pharmaceutical Biology and Biotechnology, University of Freiburg, Freiburg, Germany.
Department of Biochemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, Slovakia.
Front Microbiol. 2020 Sep 11;11:570606. doi: 10.3389/fmicb.2020.570606. eCollection 2020.
Ferric and ferrous iron is an essential transition metal for growth of many bacterial species including mycobacteria. The genomic region to from is putatively involved in iron/heme metabolism. We investigate the genes encoding the presumed two component system MSMEG_0244/MSMEG_0246, the neighboring gene and their involvement in this process. We show that purified MSMEG_0243 indeed is a heme binding protein. Deletion of / in leads to a defect in biofilm formation and colony growth on solid agar, however, this phenotype is independent of the supplied iron source. Further, analysis of the corresponding mutant and its lipids reveals that changes in morphology and biofilm formation correlate with altered acylation patterns of phosphatidylinositol mannosides (PIMs). We provide the first evidence that work in concert in cellular lipid homeostasis, especially in the maintenance of PIMs, with the heme-binding protein MSMEG_0243 as potential partner.
铁离子（三价铁和二价铁）是包括分枝杆菌在内的许多细菌生长所必需的过渡金属。从……到……的基因组区域可能参与铁/血红素代谢。我们研究了编码假定的双组分系统MSMEG_0244/MSMEG_0246的基因、其邻近基因以及它们在这一过程中的作用。我们发现纯化后的MSMEG_0243确实是一种血红素结合蛋白。在……中缺失……会导致生物膜形成和在固体琼脂上的菌落生长出现缺陷，然而，这种表型与所提供的铁源无关。此外，对相应突变体及其脂质的分析表明，形态和生物膜形成的变化与磷脂酰肌醇甘露糖苷（PIMs）的酰化模式改变相关。我们首次提供证据表明，……与血红素结合蛋白MSMEG_0243协同作用于细胞脂质稳态，特别是在维持PIMs方面，MSMEG_0243可能是其伙伴。 （注：原文中部分表述不太完整准确，翻译可能存在一定局限性）
