基于高效咔啉生物碱的探针用于小麦胚组织内源性谷胱甘肽的高灵敏度成像
Efficient -Carboline Alkaloid-Based Probe for Highly Sensitive Imaging of Endogenous Glutathione in Wheat Germ Tissues.
作者信息
Ji Xiaohui, Zhang Dan, Li Le, Jin Lingxia, Wu Rui
机构信息
Shaanxi Province Key Laboratory of Catalytic Foundation and Application, School of Chemistry and Environment Science, Shaanxi University of Technology, Hanzhong 723001, China.
Shaanxi Key Laboratory of Industrial Automation, School of Mechanical Engineering, Shaanxi University of Technology, Hanzhong 723001, China.
出版信息
Int J Anal Chem. 2020 Sep 15;2020:8675784. doi: 10.1155/2020/8675784. eCollection 2020.
Discriminative detection of GSH is achieved by employing a highly sensitive and selective fluorescent probe () that bears -carboline alkaloid as a potential fluorophore and an azide group as the recognition unit. A rapid fluorescence off-on change is caused by special redox reaction; has the capability of monitoring endogenous GSH in wheat germ tissues, indicating that this probe holds great potential for biological applications in plant tissues.
通过使用一种高灵敏度和选择性的荧光探针（）来实现对谷胱甘肽（GSH）的鉴别检测，该探针以β-咔啉生物碱作为潜在的荧光团，以叠氮基团作为识别单元。特殊的氧化还原反应会引起快速的荧光开关变化；该探针具有监测小麦胚芽组织中内源性谷胱甘肽的能力，表明这种探针在植物组织的生物应用中具有巨大潜力。
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