基于可逆反应的荧光探针用于线粒体中谷胱甘肽动力学的实时成像
Reversible Reaction-Based Fluorescent Probe for Real-Time Imaging of Glutathione Dynamics in Mitochondria.
作者信息
Chen Jianwei, Jiang Xiqian, Zhang Chengwei, MacKenzie Kevin R, Stossi Fabio, Palzkill Timothy, Wang Meng C, Wang Jin
机构信息
Department of Pharmacology and Chemical Biology, ‡Center for Drug Discovery, §Department of Molecular and Cellular Biology, ∥Integrated Microscopy Core, Advanced Technology Cores, ⊥Department of Molecular and Human Genetics, and #Huffington Center on Aging, Baylor College of Medicine , Houston, Texas 77030, United States.
出版信息
ACS Sens. 2017 Sep 22;2(9):1257-1261. doi: 10.1021/acssensors.7b00425. Epub 2017 Aug 18.
Abstract
We report a mitochondria-specific glutathione (GSH) probe-designated as Mito-RealThiol (MitoRT)-that can monitor in vivo real-time mitochondrial glutathione dynamics, and apply this probe to follow mitochondrial GSH dynamic changes in living cells for the first time. MitoRT can be utilized in confocal microscopy, super-resolution fluorescence imaging, and flow cytometry systems. Using MitoRT, we demonstrate that cells have a high priority to maintain the GSH level in mitochondria compared to the cytosol not only under normal growing conditions but also upon oxidative stress.
摘要
我们报告了一种线粒体特异性谷胱甘肽（GSH）探针——命名为线粒体实时硫醇（Mito-RealThiol，MitoRT），它可以监测体内线粒体谷胱甘肽的实时动态变化，并首次将该探针应用于追踪活细胞中线粒体谷胱甘肽的动态变化。MitoRT可用于共聚焦显微镜、超分辨率荧光成像和流式细胞术系统。使用MitoRT，我们证明，不仅在正常生长条件下，而且在氧化应激时，与细胞质相比，细胞都高度优先维持线粒体中的谷胱甘肽水平。
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