Salter A M, Fisher S C, Brindley D N
FEBS Lett. 1987 Aug 10;220(1):159-62. doi: 10.1016/0014-5793(87)80895-5.
Rat hepatocytes were maintained in monolayer culture for 20 h in the presence of 10% (v/v) newborn calf serum and then for a further 1-24 h in serum-free medium containing 2 g bovine serum albumin/l. The specific binding of human 125I-LDL to two distinct sites was then measured at 4 degrees C. Binding to site 1 was displaced by dextran sulphate while that to site 2 was not. The presence of 1-100 nM insulin for 24 h in the second incubation significantly increased binding to site 1. Significant increases were also seen when cells were incubated with 10 nM insulin for 1 h. No significant effects of insulin on binding to site 2 were observed. In contrast, 10 nM-1 microM dexamethasone decreased binding to both sites. The effects of these hormones were mutually antagonistic.
大鼠肝细胞在含有10%（体积/体积）新生牛血清的条件下进行单层培养20小时，然后在含有2 g/L牛血清白蛋白的无血清培养基中再培养1 - 24小时。随后在4℃下测定人125I - LDL与两个不同位点的特异性结合。与位点1的结合可被硫酸葡聚糖取代，而与位点2的结合则不能。在第二次孵育中，1 - 100 nM胰岛素存在24小时可显著增加与位点1的结合。当细胞与10 nM胰岛素孵育1小时时，也观察到显著增加。未观察到胰岛素对与位点2结合的显著影响。相比之下，10 nM - 1 μM地塞米松可降低与两个位点的结合。这些激素的作用相互拮抗。
