Salter A M, Fisher S C, Brindley D N
Department of Biochemistry, University of Nottingham Medical School, Queen's Medical Centre, U.K.
Atherosclerosis. 1988 May;71(1):77-80. doi: 10.1016/0021-9150(88)90304-8.
Rat hepatocytes were maintained for the first 24 h in culture in the presence of 10% (v/v) newborn calf serum and then for a further 16 h in serum-free medium containing 2 g bovine serum albumin per litre. The presence of 1-100 nM triiodothyronine (T3) in the second incubation significantly increased binding of human 125I-LDL to the LDL receptor. Unlike insulin, T3 was unable to reverse the decrease in binding brought about by dexamethasone. The increased binding to the LDL receptor produced by insulin and T3 was additive. We conclude that T3, insulin and glucocorticoids may play important roles in regulating plasma LDL concentrations by direct effect on LDL uptake by the liver.
大鼠肝细胞在含10%（体积/体积）新生牛血清的培养液中培养24小时，然后在每升含2克牛血清白蛋白的无血清培养基中再培养16小时。在第二次培养中加入1 - 100纳摩尔的三碘甲状腺原氨酸（T3）能显著增加人125I - 低密度脂蛋白（LDL）与LDL受体的结合。与胰岛素不同，T3不能逆转地塞米松引起的结合减少。胰岛素和T3对LDL受体结合的增加具有相加作用。我们得出结论，T3、胰岛素和糖皮质激素可能通过直接影响肝脏对LDL的摄取在调节血浆LDL浓度中起重要作用。
