[利用DNA条形码技术快速鉴定冬虫夏草及其制品]

[Rapid identification of Cordyceps and its products using DNA barcoding method].

作者信息

Su Yan-Yan, Li Wen-Jia, Yang Li, Ding Dan-Dan, Xiang Li

机构信息

Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.

Key Laboratory of State Administration of Traditional Chinese Medicine,Sunshine Lake Pharma Co.,LTD Dongguan 523850,China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2019 May;44(10):1965-1973. doi: 10.19540/j.cnki.cjcmm.20190131.002.

DOI:10.19540/j.cnki.cjcmm.20190131.002

PMID:31355548

Abstract

Cordyceps is one of the most valuable traditional Chinese medicines. There are various counterfeits in markets because of high price and limited output. In this study,116 Cordyceps,146 hosts and 29 related products were collected and detected by using normal DNA barcoding technology and specific PCR method. The results indicated that Cordyceps and its adulterants could be distinguished from each other through DNA barcoding technology based on ITS and COⅠsequences. Two pairs specific primers ITSSF1/ITSSR1 and ITSSF2/ITSSR2 were developed to amplify 297 bp and 136 bp ITS regions of Cordyceps sinensis,respectively. It could be used to identify C. sinensis specifically and rapidly. Furthermore,specific primers ITSSF1/ITSSR1 and ITSSF2/ITSSR2 combined with ITS and COⅠsequences could differentiate powder Cordyceps from fermentation mycelia and could identify related products. Therefore,the method developed from this study could be applied to identify the powder of Cordyceps from fermentation mycelia and related products efficiently.

摘要

冬虫夏草是最名贵的中药材之一。由于价格高昂且产量有限，市场上存在各种假冒伪劣产品。本研究采用常规DNA条形码技术和特异性PCR方法，对116份冬虫夏草、146份寄主及29份相关产品进行了收集与检测。结果表明，基于ITS和COⅠ序列的DNA条形码技术能够区分冬虫夏草及其掺假品。开发了两对特异性引物ITSSF1/ITSSR1和ITSSF2/ITSSR2，分别扩增中华虫草菌ITS区域的297 bp和136 bp片段，可用于中华虫草菌的特异性快速鉴定。此外，特异性引物ITSSF1/ITSSR1和ITSSF2/ITSSR2与ITS和COⅠ序列相结合，能够区分冬虫夏草粉末与发酵菌丝体，并鉴定相关产品。因此，本研究建立的方法可有效应用于区分冬虫夏草粉末与发酵菌丝体及相关产品。