Barreto Hugo C, Frazão Nelson, Sousa Ana, Konrad Anke, Gordo Isabel
Instituto Gulbenkian de Ciência, Oeiras, Portugal.
IBiMed, Institute for Biomedicine, Universidade de Aveiro, Aveiro, Portugal.
Commun Integr Biol. 2020 Jun 24;13(1):89-96. doi: 10.1080/19420889.2020.1783059.
The ecology and environment of the microbes that inhabit the mammalian intestine undergoes several changes as the host ages. Here, we ask if the selection pressure experienced by a new strain colonizing the aging gut differs from that in the gut of young adults. Using experimental evolution in mice after a short antibiotic treatment, as a model for a common clinical situation, we show that a new colonizing strain rapidly adapts to the aging gut via both mutation accumulation and bacteriophage-mediated horizontal gene transfer (HGT). The pattern of evolution of in aging mice is characterized by a larger number of transposable element insertions and intergenic mutations compared to that in young mice, which is consistent with the gut of aging hosts harboring a stressful and iron limiting environment.
随着宿主年龄的增长，栖息在哺乳动物肠道内的微生物的生态和环境会发生多种变化。在此，我们探究定殖于老龄肠道的新菌株所经历的选择压力是否与年轻成年人肠道中的不同。通过短期抗生素治疗后在小鼠中进行实验进化，以此作为常见临床情况的模型，我们发现新的定殖菌株通过突变积累和噬菌体介导的水平基因转移（HGT）迅速适应老龄肠道。与年轻小鼠相比，老龄小鼠中[此处原文似乎缺失相关内容]的进化模式的特征在于转座元件插入和基因间突变的数量更多，这与老龄宿主的肠道具有应激性且铁含量有限的环境一致。
