Hou Jingxia, Lin Shangyang, Lu Jinlan, Wu Yu, Wu Li, Chen Zhipeng, Li Weidong
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China.
J Anal Methods Chem. 2020 Sep 17;2020:8832736. doi: 10.1155/2020/8832736. eCollection 2020.
Qing'e pills is clinically used for treating osteoporosis in postmenopausal women in China. Eucommiae Cortex and Psoraleae Fructus are the main herbs of Qing'e pills and are both required to be salt-processed. In order to study the influence of salt-processing on the tissue distribution of Qing'e pills, a UPLC-MS/MS method was established for studying the tissue distribution of 12 main bioactive ingredients of Qing'e pills in rats. The linear relationships of the 12 compounds in each tissue were good. The method was fully validated for its selectivity, accuracy, precision, stability, matrix effect, and extraction recovery. Then, the validated method was successfully applied for simultaneous determination of the 12 chemical components in Qing'e pills in tissues for the first time. Areas under the curve (AUC) results showed that, except for pinoresinol diglucoside, psoralen, and isopsoralen, the distribution of the other components was increased in the kidney, uterus, ovary, and testes. Relative targeting efficiency (RTE) results showed that all 12 chemical components targeted the kidney and sexual organs. The results indicated that the Eucommiae Cortex and Psoraleae Fructus after salt-processing could significantly increase the distribution of components to the kidney and generative organs.
在中国，青娥丸临床上用于治疗绝经后女性的骨质疏松症。杜仲和补骨脂是青娥丸的主要药材，二者均需盐炙。为研究盐炙对青娥丸组织分布的影响，建立了一种超高效液相色谱-串联质谱法（UPLC-MS/MS），用于研究青娥丸12种主要生物活性成分在大鼠体内的组织分布。各组织中12种化合物的线性关系良好。该方法在选择性、准确性、精密度、稳定性、基质效应和提取回收率方面均得到充分验证。然后，该验证方法首次成功应用于同时测定青娥丸组织中的12种化学成分。曲线下面积（AUC）结果表明，除松脂醇二葡萄糖苷、补骨脂素和异补骨脂素外，其他成分在肾脏、子宫、卵巢和睾丸中的分布均增加。相对靶向效率（RTE）结果表明，所有12种化学成分均靶向肾脏和性器官。结果表明，盐炙后的杜仲和补骨脂可显著增加成分在肾脏和生殖器官中的分布。
