Banerjee Rajanya, Samanta Mrinal, Das Surajit
Laboratory of Environmental Microbiology and Ecology (LEnME), Department of Life Science, National Institute of Technology, Rourkela, Odisha 769008 India.
Fish Health Management Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha 751002 India.
3 Biotech. 2020 Oct;10(10):445. doi: 10.1007/s13205-020-02435-7. Epub 2020 Sep 19.
Toll-like receptors (TLRs) in innate immune system act as primary sensors in detecting the microbial components and activate their signaling cascades to induce NF-κB (nuclear factor NF-κB) towards the augmentation of immunoglobulin (Ig) synthesis. To gain insights into the efficacy of NF-κB pathway in immunoglobulin D (IgD) synthesis in the Indian Major Carp cloning and sequencing of TLR-signaling downstream molecules [TRAF3 (TNF receptor-associated factor 3), NEMO (nuclear factor-kappa B essential modulator), NF-κB and BAFF (B cell activating factor)] were performed by infecting the fish with pathogens. mRNA expression analysis of the downstream molecules and IgD showed significant up-regulation of these genes in kidney ( ≤ 0.001) as compared to spleen ( ≤ 0.05). To ascertain the role of NF-κB pathway in IgD synthesis, the primary cell culture of kidney and spleen in monolayer cell suspension was treated with NF-κB inhibitor (BAY 11-7082) and down-regulation of , , , and gene was observed. These results highlight the importance of NF-κB signaling pathway in augmenting the gene expression in the freshwater carp, .
天然免疫系统中的Toll样受体（TLRs）作为检测微生物成分的主要传感器，并激活其信号级联反应以诱导核因子κB（NF-κB），从而增强免疫球蛋白（Ig）的合成。为深入了解NF-κB途径在印度鲤免疫球蛋白D（IgD）合成中的作用，通过用病原体感染鱼类，对TLR信号下游分子[肿瘤坏死因子受体相关因子3（TRAF3）、核因子κB必需调节因子（NEMO）、NF-κB和B细胞活化因子（BAFF）]进行克隆和测序。与脾脏相比（≤0.05），对下游分子和IgD的mRNA表达分析显示，这些基因在肾脏中显著上调（≤0.001）。为确定NF-κB途径在IgD合成中的作用，用NF-κB抑制剂（BAY 11-7082）处理单层细胞悬液中的肾脏和脾脏原代细胞培养物，观察到TRAF3、NEMO、NF-κB和BAFF基因表达下调。这些结果突出了NF-κB信号通路在增强淡水鲤IgD基因表达中的重要性。
