壳聚糖包被的二氧化钛包埋紫杉醇纳米颗粒增强对骨肉瘤的抗肿瘤疗效。
Chitosan-Coated Titanium Dioxide-Embedded Paclitaxel Nanoparticles Enhance Anti-Tumor Efficacy Against Osteosarcoma.
作者信息
Qu Yang, Kang Mingyang, Cheng Xueliang, Zhao Jianwu
机构信息
Department of Orthopedics, The Second Hospital of Jilin University, Changchun, China.
出版信息
Front Oncol. 2020 Sep 9;10:577280. doi: 10.3389/fonc.2020.577280. eCollection 2020.
OBJECTIVE
Titanium dioxide nanoparticles (TiO) nanoparticles have been widely explored in the prevention of cancer risk. Due to the difficult solubility of TiO nanoparticles, it is essential to synthesize new surfactants to increase its bioavailability and anti-tumor activity and reduce its cytotoxicity. Furthermore, oxidative and inflammation are closely associated with the osteosarcoma risk. Chitosan has biocompatibility, antioxidant and anti-inflammatory properties. The effects of chitosan-coated TiO-embedded paclitaxel nanoparticles on an osteosarcoma model were explored.
METHODS
An osteosarcoma model was established and chitosan-coated TiO-embedded paclitaxel nanoparticles were prepared using a freeze-drying strategy. The morphological characteristics of nanoparticles were observed using scanning electron microscopy (SEM). The physicochemical properties of nanoparticle were evaluated by X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy. The cytotoxicity was tested by using human osteoblast cells hFob1.19 and osteosarcoma cells 143B. Osteosarcoma mice were treated with PBS buffer, paclitaxel, TiO-embedded paclitaxel and chitosan-coated TiO-embedded paclitaxel nanoparticles. The biomarkers of oxidative-inflammatory status, anti-tumor activities and survival rates of the model were measured.
RESULTS
XRD analysis showed that the peaks of chitosan/TiO (anatase) were consistent with those of crystalline TiO and broad phase of chitosan. The FTIR spectrum indicated the relevant functional groups in TiO. Chitosan-coated TiO-embedded paclitaxel nanoparticles had good biocompatibility and improve antioxidant and anti-inflammatory properties in the osteosarcoma model. Chitosan-coated TiO-embedded paclitaxel nanoparticles was less toxic to the cells hFob1.19 and more toxic to the cells 143B than TiO-embedded paclitaxel nanoparticles. Chitosan-coated TiO-embedded paclitaxel nanoparticles showed significant antitumor activity and increased the survival rate of the osteosarcoma model ( < 0.05).
CONCLUSIONS
Chitosan improved anti-tumor potential of TiO-embedded paclitaxel nanoparticles in the prevention of osteosarcoma.
目的
二氧化钛纳米颗粒（TiO）已在癌症风险预防方面得到广泛研究。由于TiO纳米颗粒溶解性差，合成新型表面活性剂以提高其生物利用度和抗肿瘤活性并降低其细胞毒性至关重要。此外，氧化和炎症与骨肉瘤风险密切相关。壳聚糖具有生物相容性、抗氧化和抗炎特性。本研究探讨了壳聚糖包被的TiO负载紫杉醇纳米颗粒对骨肉瘤模型的影响。
方法
建立骨肉瘤模型，采用冷冻干燥策略制备壳聚糖包被的TiO负载紫杉醇纳米颗粒。使用扫描电子显微镜（SEM）观察纳米颗粒的形态特征。通过X射线衍射（XRD）和傅里叶变换红外（FTIR）光谱评估纳米颗粒的物理化学性质。使用人成骨细胞hFob1.19和骨肉瘤细胞143B测试细胞毒性。用磷酸盐缓冲液（PBS）、紫杉醇、TiO负载紫杉醇和壳聚糖包被的TiO负载紫杉醇纳米颗粒处理骨肉瘤小鼠。测量模型的氧化炎症状态生物标志物、抗肿瘤活性和存活率。
结果
XRD分析表明壳聚糖/TiO（锐钛矿）的峰与结晶TiO和壳聚糖的宽峰一致。FTIR光谱表明了TiO中的相关官能团。壳聚糖包被的TiO负载紫杉醇纳米颗粒具有良好的生物相容性，并改善了骨肉瘤模型中的抗氧化和抗炎特性。与TiO负载紫杉醇纳米颗粒相比，壳聚糖包被的TiO负载紫杉醇纳米颗粒对hFob1.19细胞毒性较小，对143B细胞毒性更大。壳聚糖包被的TiO负载紫杉醇纳米颗粒显示出显著的抗肿瘤活性，并提高了骨肉瘤模型的存活率（<0.05）。
结论
壳聚糖提高了TiO负载紫杉醇纳米颗粒在预防骨肉瘤方面的抗肿瘤潜力。
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