Tsivilika Maria, Doumaki Eleni, Stavrou George, Sioga Antonia, Grosomanidis Vasilis, Meditskou Soultana, Maranginos Athanasios, Tsivilika Despina, Stafylarakis Dimitrios, Kotzampassi Katerina, Papamitsou Theodora
Faculty of Medicine, School of Health Sciences, Aristotle University of Thessaloniki, Gianni Chalkidi 45, Charilaou, 54249 Thessaloniki, Greece.
1st Department of Internal Medicine, Faculty of Medicine, AHEPA University Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece.
J Biol Res (Thessalon). 2020 Sep 29;27:15. doi: 10.1186/s40709-020-00125-2. eCollection 2020 Dec.
The present study aims to investigate, immunohistochemically, the role of the adaptive immune response in cardiac arrest/resuscitation-induced ischemia-reperfusion renal injury (IRI), namely to assess the presence of lymphocytes in renal tissue samples and the connection between the extent of the damage and the concentration of the lymphocytes by comparing the kidneys of non resuscitated swine with the kidneys of resuscitated swine.
Twenty four swine underwent cardiac arrest (CA) via a pacemaker wire. After 7 min, without any intervention, Cardiopulmonary Resuscitation, CPR, was commenced. Five min after CPR was commenced advanced life-support, ALS. Animals were divided into resuscitated animals and non resuscitated animals. Tissue samples obtained from the two groups for immunohistological study aiming to detect T-cells, B-cells and plasma cells using CD3 + , CD20 + , and CD138 + antibodies.
There seems to be a strong concentration of T lymphocytes in the kidney tissues after ischemia of both non-resuscitated and resuscitated swine. B lymphocytes, also, appear to have infiltrated the ischemic kidneys of both animal groups; nevertheless, the contribution of T lymphocytes to the induction of injury remains greater. There is no strong evidence of correlation between the plasma cells and the damage.
The adaptive immune response seems to have a strong association with kidney injury and acute tubular necrosis after cardiac arrest/ resuscitation-induced ischemia-reperfusion. However, the extent to which the adaptive immune cells are involved in the induction of renal injury remains uncertain and there are many questions about the mechanism of function of these cells, the answers of which require further studies.
本研究旨在通过免疫组织化学方法研究适应性免疫反应在心脏骤停/复苏诱导的缺血再灌注肾损伤（IRI）中的作用，即通过比较未复苏猪和复苏猪的肾脏，评估肾组织样本中淋巴细胞的存在情况以及损伤程度与淋巴细胞浓度之间的关系。
24只猪通过起搏导线诱导心脏骤停（CA）。7分钟后，在未进行任何干预的情况下开始心肺复苏（CPR）。CPR开始5分钟后进行高级生命支持（ALS）。动物被分为复苏组和未复苏组。从两组获取组织样本进行免疫组织学研究，旨在使用CD3 +、CD20 +和CD138 +抗体检测T细胞、B细胞和浆细胞。
未复苏和复苏猪缺血后肾组织中似乎都有大量T淋巴细胞聚集。B淋巴细胞也似乎浸润了两组动物的缺血肾脏；然而，T淋巴细胞对损伤诱导的作用仍然更大。没有强有力的证据表明浆细胞与损伤之间存在相关性。
适应性免疫反应似乎与心脏骤停/复苏诱导的缺血再灌注后的肾损伤和急性肾小管坏死密切相关。然而，适应性免疫细胞参与肾损伤诱导的程度仍不确定，关于这些细胞的功能机制有许多问题，其答案需要进一步研究。
