Stem Cell Center, Department of Biology and Biochemistry, University of Houston, Houston, TX 77204, USA.
Technical Contact.
STAR Protoc. 2020 Sep 18;1(2). doi: 10.1016/j.xpro.2020.100063. Epub 2020 Jul 9.
The plurality of clonogenic cells derived from human lung includes a spectrum of diverse p63+ stem cells responsible for the regeneration of normal epithelial tissue and disease-associated metaplastic lesions. Here, we report protocols for the cloning, expansion, and characterization of these stem cell variants, which in general assist in analyses of stem cell heterogeneity, genome editing, drug screening, and regenerative medicine. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2011), Zuo et al. (2015), and Rao et al. (2020).
从人肺中分离的克隆形成细胞的多样性包括一系列不同的 p63+干细胞，这些干细胞负责正常上皮组织的再生和与疾病相关的化生病变。在这里，我们报告了这些干细胞变体的克隆、扩增和鉴定方案，这些方案通常有助于分析干细胞异质性、基因组编辑、药物筛选和再生医学。有关该方案使用和执行的完整详细信息，请参阅 Kumar 等人（2011 年）、Zuo 等人（2015 年）和 Rao 等人（2020 年）。
