Department of Nuclear Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an Shaanxi, China.
Eur Rev Med Pharmacol Sci. 2020 Sep;24(18):9473-9479. doi: 10.26355/eurrev_202009_23032.
To explore the clinical significance of circ-MTHFD2 in the diagnosis, pathological staging, and prognosis of non-small cell lung cancer (NSCLC).
A total of 100 pairs of cancer tissues and adjacent tissues were surgically removed from NSCLC patients treated from July 2011 to January 2013 in our hospital were selected. All tissue samples were pathologically confirmed. Real Time-Polymerase Chain Reaction (qRT-PCR) was adopted to detect the expression of circ-MTHFD2 in NSCLC samples and its characteristic as a circular ribonucleic acid (circRNA). The receiver operating characteristic (ROC) curve was drawn to determine the diagnostic potential of circ-MTHFD2 in NSCLC. The relationship between circ-MTHFD2 expression and the clinical characteristics of NSCLC patients was analyzed by the χ2-test. Kaplan-Meier survival curves were depicted to assess the prognostic potential of circ-MTHFD2 in NSCLC. The effect of circ-MTHFD2 on the overall survival rate of NSCLC patients was uncovered by introducing the Cox proportional hazards model.
The expression of circ-MTHFD2 in cancer tissues of NSCLC patients was higher than that in adjacent tissues (p<0.05), and there was no remarkable difference in the expression of circ-MTHFD2 before and after RNase R digestion (p>0.05). The area under the curve (AUC) of circ-MTHFD2 ROC was 0.701, with the cut-off value of 3.534, 90% sensitivity and 71% specificity. Circ-MTHFD2 expression was closely associated with smoking history, tumor size, tumor-node-metastasis (TNM) stage, lymph node metastasis, and recurrence in NSCLC patients (p<0.05). The prognosis of NSCLC patients with high expression of circ-MTHFD2 was evidently poorer than those with low expression. High expression of circ-MTHFD2 was an independent risk factor affecting the prognosis in NSCLC (p<0.05).
The high expression of circ-MTHFD2 has clinical significance in the diagnosis, pathological staging, and prognosis of NSCLC.
探讨环状 RNA 甲基四氢叶酸脱氢酶 2（circ-MTHFD2）在非小细胞肺癌（NSCLC）诊断、病理分期和预后中的临床意义。
选取我院 2011 年 7 月至 2013 年 1 月收治的 100 对 NSCLC 患者手术切除的癌组织及癌旁组织，所有组织标本均经病理证实。采用实时聚合酶链反应（qRT-PCR）检测 NSCLC 样本中 circ-MTHFD2 的表达及其作为环状核糖核酸（circRNA）的特征。绘制受试者工作特征（ROC）曲线，以确定 circ-MTHFD2 在 NSCLC 中的诊断潜力。采用 χ2 检验分析 circ-MTHFD2 表达与 NSCLC 患者临床特征的关系。采用 Kaplan-Meier 生存曲线评估 circ-MTHFD2 在 NSCLC 中的预后潜力。采用 Cox 比例风险模型揭示 circ-MTHFD2 对 NSCLC 患者总生存率的影响。
NSCLC 患者癌组织中 circ-MTHFD2 的表达高于癌旁组织（p<0.05），且经 RNase R 消化前后 circ-MTHFD2 的表达无显著差异（p>0.05）。circ-MTHFD2 的 ROC 曲线下面积（AUC）为 0.701，以 3.534 为截断值，其灵敏度为 90%，特异性为 71%。circ-MTHFD2 表达与 NSCLC 患者的吸烟史、肿瘤大小、肿瘤-淋巴结-转移（TNM）分期、淋巴结转移和复发密切相关（p<0.05）。circ-MTHFD2 高表达的 NSCLC 患者的预后明显差于低表达的患者。circ-MTHFD2 高表达是影响 NSCLC 预后的独立危险因素（p<0.05）。
circ-MTHFD2 的高表达在 NSCLC 的诊断、病理分期和预后中具有临床意义。
