College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China.
Marshall Laboratory of Biomedical Engineering, International Cancer Center, Laboratory of Evolutionary Theranostics (LET), School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen, 518060, China.
Adv Mater. 2020 Dec;32(48):e2004481. doi: 10.1002/adma.202004481. Epub 2020 Oct 5.
Simultaneous photodynamic therapy (PDT) and photothermal therapy (PTT) can reduce the risks of drug leakage, body burden, and preparation complexity in traditional combination PDT/PTT. Here, a versatile nanoporphyrin (Pp18-lipos) self-assembled from lipid-purpurin 18 conjugates (Pp18-lipids) and pure lipids is presented. The as-prepared Pp18-lipos with 2 mol% Pp18-lipids can perform effective PDT and fluorescence imaging. The Pp18-lipos with 65 mol% Pp18 can perform potent PTT and photoacoustic imaging. The chelation of Mn endows the Pp18-lipids-Mn a high T -weighted magnetic resonance imaging contrast. Notably, pretreatment of low-dose PDT facilitates the endocytosis and tumor accumulation of Pp18-lipos, thus achieving synergistic PDT/PTT. Upon exposure to a single 705 nm-laser, the combination of PDT/PTT achieves a significantly higher tumor growth inhibition rate than PDT or PTT alone. In addition, it is found that the synergistic PDT/PTT triggers more potent anti-tumor immune response including tumor infiltration of immune cells and release of related cytokines.
同时的光动力疗法 (PDT) 和光热疗法 (PTT) 可以降低传统联合 PDT/PTT 中药物泄漏、身体负担和准备复杂性的风险。在这里，提出了一种由脂质卟啉 18 缀合物 (Pp18-lipids) 和纯脂质自组装而成的多功能纳米卟啉 (Pp18-lipos)。制备的 Pp18-lipos 中含有 2 mol% Pp18-lipids 可进行有效的 PDT 和荧光成像。含有 65 mol% Pp18 的 Pp18-lipos 可进行有效的 PTT 和光声成像。Mn 的螯合赋予了 Pp18-lipids-Mn 高 T1 加权磁共振成像对比。值得注意的是，低剂量 PDT 的预处理促进了 Pp18-lipos 的内吞作用和肿瘤积累，从而实现协同的 PDT/PTT。在单次 705nm 激光照射下，PDT/PTT 的联合治疗比单独 PDT 或 PTT 实现了更高的肿瘤生长抑制率。此外，研究发现协同的 PDT/PTT 引发了更强的抗肿瘤免疫反应，包括免疫细胞浸润和相关细胞因子的释放。
